Prognostic Significance of Lymphatic Vessel Density by D2-40 Immune Marker and Mast Cell Density in Invasive Breast Cancer: A Cross Sectional Study at Tertiary Care Hospital in South India.

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dc.contributor.authorDhanlakshmi, Ben_US
dc.contributor.authorAmita, Ken_US
dc.contributor.authorPrashantha, K.en_US
dc.date.accessioned2023-07-14T06:58:54Z
dc.date.available2023-07-14T06:58:54Z
dc.date.issued2022-03
dc.description.abstractBackground: Tumour induced lymphangiogenesis plays a crucial role in metastasis and tumour progression. The intratumoural and peritumoral lymphatics are supposed to have different biological effects. The aim of present study was to investigate the correlation of intratumoral lymphatic vessel density (I LVD), peritumoral lymphatic vessel density (P LVD), intratumoral mast cell density (I MCD) and peritumoral mast cell density (P MCD) with prognostic parameters in primary breast carcinoma. Methods: Lymphangiogenesis was detected using D2-40 monoclonal antibody and mast cell by using toludine blue stain in 50 cases of primary breast carcinoma. Positively stained lymphatic vessels were counted at 40 x in dense lymphatic vascular foci (hot-spot) within the tumour. Chi square, ANOVA test and Pearsons correlation was applied to determine the relationship amongst various variables, with statistical significance set at p <0.05. Results: Mean P LVD was significantly higher than I LVD (6.25±21 vs 2.75±2.27,p <0.005). Significant correlation was noted between I LVD and P LVD and age, tumour laterality, tumour size and overall staging. However, there was no correlation between I LVD and P LVD with other important clinicopathologic prognostic markers like grade, lymphnode status and lymphovascular invasion. MCD was higher in both intratumoral and peritumoral location as compared to normal tissue. There was an association noted between P MCD with pathological staging and perineural invasion. However, there was no significant association of I MCD and P MCD with other prognostic markers like grade and lymphnode status. No significant correlation was noted between I LVD, P LVD, I MCD and P MCD. Conclusion: The evidences from our study support the utility of D2-40 stain in determining the lymphatic density in IBC. The study findings also establish the existence of lymphangiogenesis in both intratumoral and peritumoral location. For now, the data presented herein do not permit us to promote the utility of LVD and MCD as predictors of prognosis in invasive breast carcinoma.en_US
dc.identifier.affiliationsAdichunchanagiri University, B.G.Nagara, Mandya District, Karnataka 571448, India.en_US
dc.identifier.citationDhanlakshmi B, Amita K, Prashantha K.. Prognostic Significance of Lymphatic Vessel Density by D2-40 Immune Marker and Mast Cell Density in Invasive Breast Cancer: A Cross Sectional Study at Tertiary Care Hospital in South India.. Light House Polyclinic. 2022 Mar; 21(1): 1-10en_US
dc.identifier.issn0972-5997
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/218336
dc.languageenen_US
dc.publisherLight House Polyclinicen_US
dc.relation.issuenumber1en_US
dc.relation.volume21en_US
dc.source.urihttps://www.ojhas.org/issue81/2022-1-5.pdfen_US
dc.subjectMast cellen_US
dc.subjectLymphangiogenesisen_US
dc.subjectBreast canceren_US
dc.subjectPrognostic factorsen_US
dc.titlePrognostic Significance of Lymphatic Vessel Density by D2-40 Immune Marker and Mast Cell Density in Invasive Breast Cancer: A Cross Sectional Study at Tertiary Care Hospital in South India.en_US
dc.typeJournal Articleen_US
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