Western blot analysis of serological response in Helicobacter pylori in acid peptic diseases.

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dc.contributor.authorChatha, Len_US
dc.contributor.authorRay, Pen_US
dc.contributor.authorBhasin, D Ken_US
dc.contributor.authorPanigrahi, Den_US
dc.contributor.authorKhanna, Ten_US
dc.contributor.authorVaiphei, Ken_US
dc.contributor.authorSingh, Ken_US
dc.contributor.authorSingh, Hen_US
dc.date.accessioned1997-04-01en_US
dc.date.accessioned2009-05-27T07:17:31Z
dc.date.available1997-04-01en_US
dc.date.available2009-05-27T07:17:31Z
dc.date.issued1997-04-01en_US
dc.description.abstractA prospective study was undertaken in 70 patients presenting with acid peptic disease with the objective of characterising the serological response to Helicobacter pylori and finding antigens specific for the serodiagnosis of H. pylori infection. H. pylori status was assessed by smear microscopy, rapid urease activity, culture and histopathology of endoscopic gastric antral biopsy specimens. Serological characterisation was carried out by using western blotting of various antigenic components of H. pylori and subsequent enzymatic detection of antibodies against them. Four reactive bands in the molecular weight range of 45-65 kDa were present in all subjects irrespective of H. pylori status. Four to six immunoreactive bands in the molecular weight range of 21-45 kDa were found only in patients with positive H. pylori status and histopathologically proven gastritis. These immunoreactive components may be valuable in specific immunodiagnosis of H. pylori infection.en_US
dc.description.affiliationDepartment of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh.en_US
dc.identifier.citationChatha L, Ray P, Bhasin DK, Panigrahi D, Khanna T, Vaiphei K, Singh K, Singh H. Western blot analysis of serological response in Helicobacter pylori in acid peptic diseases. Indian Journal of Medical Research. 1997 Apr; 105(): 170-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/20100
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntigens, Bacterial --blooden_US
dc.subject.meshBiopsyen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFemaleen_US
dc.subject.meshHelicobacter Infections --immunologyen_US
dc.subject.meshHelicobacter pylori --immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune Seraen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeptic Ulcer --immunologyen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshSerologic Testsen_US
dc.titleWestern blot analysis of serological response in Helicobacter pylori in acid peptic diseases.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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