Detection of Antibiotic Resistance and Biofilm-Producing Ability of Staphylococcus Species in Clinical Isolates

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dc.contributor.authorSingh, Shila Kumarien_US
dc.contributor.authorBhattacharjee, Minakshien_US
dc.contributor.authorUnni, Balagopalanen_US
dc.contributor.authorKashyap, Rajpal Singhen_US
dc.date.accessioned2023-07-21T11:28:15Z
dc.date.available2023-07-21T11:28:15Z
dc.date.issued2023-04
dc.description.abstractBackground: Staphylococci are responsible for life-threatening infections in hospitals and community. Their ability to produce multiple virulence factors and antibiotic resistance is an important reason of high mortality in staphylococcal infections. Biofilm production by these organisms makes it difficult to treat. Most of the treating antibiotics are failing and making it a matter of concern. Aims?This study aims to detect the increased antibiotic resistance in biofilm-producing Staphylococcus and to compare the performance of three potential methods of detection. Methods?A total of 81 isolates of staphylococci including coagulase negative staphylococci (CoNs), methicillin resistant S. aureus (MRSA), and methicillin sensitive S. aureus (MSSA) are included in this study. After the identification, an antibiotic sensitivity test was performed. Biofilm detection was done by three different methods: Congo red agar method, tube adherence method, and microtiter plate method. Result?Out of the 81 samples, 37 CoNs, 17 MRSA, and 27 MSSA were identified. Out of them we got 43 (53%) biofilm producers by Congo red agar method, 40 (49%) by tube adherence method, and 52 (64%) producers by tissue culture plate/microtiter plate method. Most of the biofilm producers showed multiple drug resistance. Conclusion?We found out that the microtiter plate method is sensitive and reliable as compared with the other two methods. Antibiotic resistance was found to be very common in biofilm producers. This was due to the resistance developed as a result of the matrix that does not let the antibiotic bind with the organisms. This can make the treatment of Staphylococcus very difficult in the future as the rate of drug resistance is faster as compared with newly emerging antibiotics.en_US
dc.identifier.affiliationsDepartment of Microbiology, Faculty of Sciences, Assam Downtown University, Guwahati, Assam, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Faculty of Sciences, Assam Downtown University, Guwahati, Assam, Indiaen_US
dc.identifier.affiliationsDepartment of Research, Assam Downtown University, Guwahati, Assam, Indiaen_US
dc.identifier.affiliationsDepartment of Research, Central India Institute of Medical Science, Nagpur, Maharashtra, Indiaen_US
dc.identifier.citationSingh, Shila Kumarien_US
dc.identifier.citationBhattacharjee, Minakshien_US
dc.identifier.citationUnni, Balagopalanen_US
dc.identifier.citationKashyap, Rajpal Singh. Detection of Antibiotic Resistance and Biofilm-Producing Ability of Staphylococcus Species in Clinical Isolates. Annals of the National Academy of Medical Sciences. 2023 Apr; 58(2): 104-108en_US
dc.identifier.issn0379-038X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/220212
dc.languageenen_US
dc.publisherThieme Medical and Scientific Publishers Pvt. Ltden_US
dc.relation.issuenumber2en_US
dc.relation.volume59en_US
dc.source.urihttps://doi.org/10.1055/s-0043-1768461en_US
dc.subjectbiofilmen_US
dc.subjectantibiotic resistanceen_US
dc.subjectMRSAen_US
dc.subjectMSSAen_US
dc.subjectCoNsen_US
dc.titleDetection of Antibiotic Resistance and Biofilm-Producing Ability of Staphylococcus Species in Clinical Isolatesen_US
dc.typeJournal Articleen_US
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