Exogenous application of putrescine at pre-anthesis enhances the thermotolerance of wheat (Triticum aestivum L.).

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dc.contributor.authorKumar, Ranjeet R
dc.contributor.authorSharma, Sushil K
dc.contributor.authorRai, Gyanendra K
dc.contributor.authorSingh, Khushboo
dc.contributor.authorChoudhury, Madhumanthi
dc.contributor.authorDhawan, Gaurav
dc.contributor.authorSingh, Gyaneshwar P
dc.contributor.authorGoswami, Suneha
dc.contributor.authorPathak, Himanshu
dc.contributor.authorRai, Raj D
dc.date.accessioned2014-12-29T09:16:15Z
dc.date.available2014-12-29T09:16:15Z
dc.date.issued2014-10
dc.description.abstractAntioxidant enzymes, besides being involved in various developmental processes, are known to be important for environmental stress tolerance in plants. In this study, the effect of treatment of 2.5 mM putrescine (Put), heat stress (HS -42°C for 2 h) and their combination on the expression and activity of antioxidant enzymes was studied at pre-anthesis in the leaves of two wheat (Triticum aestivum L.) cultivars — HDR77 (thermotolerant) and HD2329 (thermosusceptible). We observed that 2.5 mM Put before HS significantly enhanced the transcript levels of superoxide dismutase (SOD), catalase (CAT), cytoplasmic and peroxisomal ascorbate peroxidase (cAPX, pAPX) in both the cultivars. However, the activities of antioxidant enzymes (SOD, CAT, APX and GR), as well as accumulation of antioxidants (ascorbic acid and total thiol content) were higher in HDR77 than in HD2329 in response to the treatment 2.5 mM Put + HS. No significant change was observed in the proline accumulation in response to HS and combined treatment of 2.5 mM Put + HS. A decrease in the H2O2 accumulation, lipid peroxidation and increase in cell membrane stability (CMS) were observed in response to 2.5 mM Put + HS treatment, as compared to HS treatment alone in both the cultivars; HDR77 was, however, more responsive to 2.5 mM Put + HS treatment. Put (2.5 mM) treatment at pre-anthesis thus modulated the defense mechanism responsible for the thermotolerance capacity of wheat under the heat stress. Elicitors like Put, therefore, need to be further studied for temporarily manipulating the thermotolerance capacity of wheat grown under the field conditions in view of the impending global climate change.en_US
dc.identifier.citationKumar Ranjeet R, Sharma Sushil K, Rai Gyanendra K, Singh Khushboo, Choudhury Madhumanthi, Dhawan Gaurav, Singh Gyaneshwar P, Goswami Suneha, Pathak Himanshu, Rai Raj D. Exogenous application of putrescine at pre-anthesis enhances the thermotolerance of wheat (Triticum aestivum L.). Indian Journal of Biochemistry & Biophysics. 2014 Oct; 51(5): 396-406.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/154269
dc.language.isoenen_US
dc.publisherNISCAIR CSIR India.en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29888en_US
dc.subjectHeat stressen_US
dc.subjectAntioxidantsen_US
dc.subjectPutrescineen_US
dc.subjectWheaten_US
dc.subjectAntioxidant enzymesen_US
dc.subjectQuantitative real-time PCRen_US
dc.subjectHeat shock proteinsen_US
dc.subjectTriticum aestivum L.en_US
dc.subject.meshHeat-Shock Proteins --chemistry
dc.subject.meshHeat-Shock Response
dc.subject.meshOxidative Stress --drug effects
dc.subject.meshOxidative Stress --physiology
dc.subject.meshPlant Leaves --drug effects
dc.subject.meshPlant Leaves --physiology
dc.subject.meshPutrescine --administration & dosage
dc.subject.meshReactive Oxygen Species --metabolism
dc.subject.meshTriticum --drug effects
dc.subject.meshTriticum --physiology
dc.titleExogenous application of putrescine at pre-anthesis enhances the thermotolerance of wheat (Triticum aestivum L.).en_US
dc.typeArticleen_US
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