Heat stable antimicrobial activity of Burkholderia gladioli OR1 against clinical drug resistant isolates.

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dc.contributor.authorBharti, Pratibha
dc.contributor.authorAnand, Vivek
dc.contributor.authorChander, Jagdish
dc.contributor.authorSingh, Inder Pal
dc.contributor.authorSingh, Tej Vir
dc.contributor.authorTewari, Rupinder
dc.date.accessioned2012-08-13T05:00:52Z
dc.date.available2012-08-13T05:00:52Z
dc.date.issued2012-05
dc.description.abstractBackground & objectives: Drug resistant microbes are a serious challenge to human health. During the search for novel antibiotics/inhibitors from the agricultural soil, a bacterial colony was found to inhibit the growth of clinical isolates including Staphylococcus (resistant to amikacin, ciprofloxacin, clindamycin, clinafloxacin, erythromycin, gentamicin and methicillin) and Candida (resistant to fluconazole and itraconazole). The culture was identified as Burkholderia gladioli and produced at least five different antimicrobial compounds which were highly stable at high temperature (121°C) and in the broad pH range (3.0-11.0). We report here the antimicrobial activity of B. gladioli against drug resistant bacterial pathogens. Methods: The bacterial culture was identified using morphological, biochemical and 16S rRNA gene sequencing techniques. The antimicrobial activity of the identified organism against a range of microbial pathogens was checked by Kirby-Bauer's disc diffusion method. The antimicrobial compounds in the cell free supernatant were chloroform-extracted and separated by thin layer chromatography (TLC). Results: B. gladioli OR1 exhibited broad spectrum antimicrobial activity against drug resistant clinical isolates belonging to various genera of bacteria (Staphylococcus, Enterobacter, Enterococcus, Acinetobacter and Citrobacter) and a fungus (Candida). Based on TLC profile and bioautography studies, the chloroform extract of B. gladioli OR1 consisted of at least three anti-staphylococcal and two anti-Candida metabolites. The antimicrobial activity was heat stable (121°C/20 min) as well as pH stable (3.0-11.0). Interpretation & conclusions: The bacterial soil isolate, B. gladioli OR1 possessed the ability to kill various drug resistant bacteria and a fungus. This organism produced many antimicrobial metabolites which might have the potential to be used as antibiotics in future.en_US
dc.identifier.citationBharti Pratibha, Anand Vivek, Chander Jagdish, Singh Inder Pal, Singh Tej Vir, Tewari Rupinder. Heat stable antimicrobial activity of Burkholderia gladioli OR1 against clinical drug resistant isolates. Indian Journal of Medical Research. 2012 May; 135(5): 666-671.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/140260
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401698/en_US
dc.subjectAntimicrobial activityen_US
dc.subjectBurkholderia gladioli OR1en_US
dc.subjectCandidaen_US
dc.subjectchloroform extracten_US
dc.subjectStaphylococcusen_US
dc.subject.meshBurkholderia gladioli --metabolism
dc.subject.meshCandida
dc.subject.meshChloroform
dc.subject.meshDrug Resistance, Multiple
dc.subject.meshAnti-Bacterial Agents
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshStaphylococcus
dc.titleHeat stable antimicrobial activity of Burkholderia gladioli OR1 against clinical drug resistant isolates.en_US
dc.typeArticleen_US
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