Rapid diagnosis of Helicobacter pylori infection in gastric imprint smears.

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dc.contributor.authorKaur, Gurjeeten_US
dc.contributor.authorMadhavan, Manoharanen_US
dc.contributor.authorBasri, Amir Hakimen_US
dc.contributor.authorSain, Abdul Hamid Maten_US
dc.contributor.authorHussain, Mohd Shaiful Bahrunen_US
dc.contributor.authorYatiban, Mohd Kamalen_US
dc.contributor.authorNaing, Nyi Nyien_US
dc.date.accessioned2009-05-27T16:23:35Z
dc.date.available2009-05-27T16:23:35Z
dc.date.issued2004-09-04en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe objective of this study was to determine the sensitivity, specificity, positive (PPV), and negative predictive values (NPV) of Diff-Quik-stained gastric imprint cytology smears in the detection of H. pylori compared with histology. Air-dried imprint smears of gastric biopsies from 150 patients were stained by the Diff-Quik method in the endoscopy suite and examined for H. pylori, providing results within minutes. The presence of inflammation and intestinal metaplasia were documented. The same biopsy was processed and stained with H&E and Warthin-Starry stains, and reviewed by a different pathologist blind to the imprint cytology results. Ninety-four of the 150 patients were male with a mean age of 50 years. Based on histology, the H. pylori prevalence was very low at 8%. The sensitivity and specificity of imprint cytology in the detection of H. pylori were 83.3% and 100%, respectively. The PPV and NPV were 100% and 98.6%, respectively. There were two false negatives and no false positives. A combination of imprint cytology and histology achieved 100% sensitivity. Imprint smears did not provide added value over histology with regards to inflammation and metaplasia. Gastric imprint smears stained with Diff-Quik method is a rapid, cheap, and reliable method for the detection of H. pylori and have their best results when complemented with histology.en_US
dc.description.affiliationDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia. gurjeet@notes.usm.myen_US
dc.identifier.citationKaur G, Madhavan M, Basri AH, Sain AH, Hussain MS, Yatiban MK, Naing NN. Rapid diagnosis of Helicobacter pylori infection in gastric imprint smears. The Southeast Asian Journal of Tropical Medicine and Public Health. 2004 Sep; 35(3): 676-80en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35105
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2004_35_3/31-3253.pdfen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 and overen_US
dc.subject.meshAzure Stains --diagnostic useen_US
dc.subject.meshBiopsyen_US
dc.subject.meshChilden_US
dc.subject.meshCytodiagnosis --methodsen_US
dc.subject.meshEndoscopy, Digestive Systemen_US
dc.subject.meshFemaleen_US
dc.subject.meshHelicobacter Infections --pathologyen_US
dc.subject.meshHelicobacter pylori --isolation & purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMethylene Blue --diagnostic useen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshPyloric Antrum --microbiologyen_US
dc.subject.meshStaining and Labeling --methodsen_US
dc.subject.meshXanthenes --diagnostic useen_US
dc.titleRapid diagnosis of Helicobacter pylori infection in gastric imprint smears.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeValidation Studiesen_US
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