Distribution of Chlamydia trachomatis omp A genotypes in patients attending a sexually transmitted disease outpatient clinic in New Delhi, India

Simple item page
dc.contributor.authorRawre, Jen_US
dc.contributor.authorDhawan, Ben_US
dc.contributor.authorKhanna, Nen_US
dc.contributor.authorSreenivas, Ven_US
dc.contributor.authorBroor, Sen_US
dc.contributor.authorChaudhry, Ren_US
dc.date.accessioned2020-04-10T01:42:37Z
dc.date.available2020-04-10T01:42:37Z
dc.date.issued2019-05
dc.description.abstractBackground & objectives: Limited data are available on the typing of Chlamydia trachomatis in India. Serovars D to K of C. trachomatis are chiefly responsible for urogenital infections. Thus, this study was conducted to determine the distribution of C. trachomatis serovars in patients with urogenital infections and to characterize omp A gene of the detected C. trachomatis isolates by sequence analysis. Presence of other co-infections was also evaluated. Methods: Endocervical swabs were collected from 324 women and urethral swabs/urine were collected from 193 men attending the sexually transmitted diseases outpatient clinic. The samples were screened for C. trachomatis by cryptic plasmid PCR and omp A gene PCR. Genotyping was performed by PCR-restriction fragment length polymorphism (RFLP) and sequencing of the omp A gene. Samples were screened for genital mycoplasmas, Neisseria gonorrhoeae, Treponema pallidum and human immunodeficiency virus (HIV). Results: C. trachomatis was found in 15.0 per cent men and 10.8 per cent women. Serovar D was the most prevalent followed by serovars E, F, I and G. Twenty two C. trachomatis isolates were selected for omp A gene sequencing. No mixed infection was found. Variability in omp A sequences was seen in 31.8 per cent cases. Both PCR-RFLP and omp A gene sequencing showed concordant results. The presence of Ureaplasma spp. and Mycoplasma hominis was observed in 18.7 and 9.5 per cent patients, respectively. Co-infection of C. trachomatis was significantly associated with Ureaplasma urealyticum and HIV. Interpretation & conclusions: The high occurence of C. trachomatis infections warrants its screening in addition to other sexually transmitted infections namely U. urealyticum and HIV. Genotyping of the omp A gene may provide additional information for vaccine development.en_US
dc.identifier.affiliationsDepartment of Microbiology, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Dermatology and Venereology, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Biostatistics, All India Institute of Medical Sciences, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology, SGT Medical College, Gurugram, Indiaen_US
dc.identifier.citationRawre J, Dhawan B, Khanna N, Sreenivas V, Broor S, Chaudhry R. Distribution of Chlamydia trachomatis omp A genotypes in patients attending a sexually transmitted disease outpatient clinic in New Delhi, India. Indian Journal of Medical Research. 2019 May; 149(5): 662-670en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195876
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber5en_US
dc.relation.volume149en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1171_17en_US
dc.subjectChlamydia trachomatis- omp A geneen_US
dc.subjectPCR-RFLPen_US
dc.subjectsequencingen_US
dc.subjectserovarsen_US
dc.titleDistribution of Chlamydia trachomatis omp A genotypes in patients attending a sexually transmitted disease outpatient clinic in New Delhi, Indiaen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijmr2019v149n5p662.pdf
Size:
709.11 KB
Format:
Adobe Portable Document Format
Download
Collections
Indian Journal of Medical Research