Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.

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dc.contributor.authorBoonsaeng, Ven_US
dc.contributor.authorChansiri, Ken_US
dc.contributor.authorVilasineekul, Pen_US
dc.contributor.authorWilairat, Pen_US
dc.contributor.authorPanyim, Sen_US
dc.date.accessioned2009-05-27T16:12:23Z
dc.date.available2009-05-27T16:12:23Z
dc.date.issued1989-12-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractA simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.en_US
dc.description.affiliationDepartment of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationBoonsaeng V, Chansiri K, Vilasineekul P, Wilairat P, Panyim S. Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application. The Southeast Asian Journal of Tropical Medicine and Public Health. 1989 Dec; 20(4): 519-22en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34669
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDNA Probesen_US
dc.subject.meshEpidemiologic Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshMalaria --blooden_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPhosphorus Radioisotopesen_US
dc.subject.meshPlasmodium falciparum --isolation & purificationen_US
dc.titleDetection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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