Autophagy requires Tip20 in Saccharomyces cerevisiae

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dc.contributor.authorChen, Leien_US
dc.contributor.authorZhang, Chunlingen_US
dc.contributor.authorLiang, Yuancunen_US
dc.contributor.authorLiu, Aixinen_US
dc.contributor.authorDong, Hansongen_US
dc.contributor.authorZou, Shenshenen_US
dc.date.accessioned2020-11-18T10:14:15Z
dc.date.available2020-11-18T10:14:15Z
dc.date.issued2019-03
dc.description.abstractAutophagy is a highly conserved intracellular degradation pathway in eukaryotic cells that responds to environmentalchanges. Genetic analyses have shown that more than 40 autophagy-related genes (ATG) are directly involved in thisprocess in fungi. In addition to Atg proteins, most vesicle transport regulators are also essential for each step of autophagy.The present study showed that one Endoplasmic Reticulum protein in Saccharomyces cerevisiae, Tip20, which controlsGolgi-to-ER retrograde transport, was also required for starvation-induced autophagy under high temperature stress. Intip20 conditional mutant yeast, the transport of Atg8 was impaired during starvation, resulting in multiple Atg8 punctadispersed outside the vacuole that could not be transported to the pre-autophagosomal structure/phagophore assembly site(PAS). Several Atg8 puncta were trapped in ER exit sites (ERES). Moreover, the GFP-Atg8 protease protection assayindicated that Tip20 functions before autophagosome closure. Furthermore, genetic studies showed that Tip20 functionsdownstream of Atg5 and upstream of Atg1, Atg9 and Atg14 in the autophagy pathway. The present data show that Tip20,as a vesicle transport regulator, has novel roles in autophagyen_US
dc.identifier.affiliationsDepartment of Plant Pathology, Shandong Agricultural University, Tai’an 271018, Chinaen_US
dc.identifier.citationChen Lei, Zhang Chunling, Liang Yuancun, Liu Aixin, Dong Hansong, Zou Shenshen. Autophagy requires Tip20 in Saccharomyces cerevisiae. Journal of Biosciences. 2019 Mar; 44(1): 1-8en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214228
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.issuenumber1en_US
dc.relation.volume44en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-018-9839-1en_US
dc.subjectAutophagyen_US
dc.subjectAtg8en_US
dc.subjectautophagosome biogenesisen_US
dc.subjectERESen_US
dc.subjectTip20en_US
dc.titleAutophagy requires Tip20 in Saccharomyces cerevisiaeen_US
dc.typeJournal Articleen_US
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