Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 3 virus, Bangkok genotype.

Simple item page
dc.contributor.authorAttatippaholkun, W Hen_US
dc.contributor.authorAttatippaholkun, M Ken_US
dc.contributor.authorNisalak, Aen_US
dc.contributor.authorVaughn, D Wen_US
dc.contributor.authorInnis, B Len_US
dc.date.accessioned2009-05-27T16:41:15Z
dc.date.available2009-05-27T16:41:15Z
dc.date.issued1998-06-14en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe nucleotide sequence of the nonstructural protein gene (1,610 bases) of dengue 3 virus (Bangkok genotype; CH53489 isolated in 1973) has been determined in both forward and reverse directions. The PCR based cycle sequencing technic by the enzymatic method of Sanger et al using a sequencing primer 5'-end labeled with gamma-32P-ATP was the method of our choice for sequence analysis. Two cDNA templates were prepared by RT-PCR technique starting from the nucleotides 6,306-6,969 and 6,925-7,915 of the dengue 3 genome with the lengths of 663 and 990 base pairs respectively. In our cycle sequencing experiments, it has been observed that the substitution of 7-deaza-dG for dG in DNA eliminated most of the secondary structures that produce gel artifacts. The final sequence results of these two cDNA templates were established from their sequence data determined on both strands in opposite directions. Alignment between the newly established nucleotide sequences as well as their deduced amino acid sequences of the Bangkok dengue 3 (CH53489) virus and the published sequence data of the dengue 3 prototype (H87) was manipulated by the PC-DOS-GIBIO DNASIS TM 06-00 software. The homology of the nucleotide sequences between the two dengue 3 viruses was 96.65%. The deduced amino acid sequence from nucleotides 6,306-7,915 of the two viruses showed conserved amino acids of the nonstructural protein NS4a and 6 amino acid changes in NS4b and NS5.en_US
dc.description.affiliationDepartment of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationAttatippaholkun WH, Attatippaholkun MK, Nisalak A, Vaughn DW, Innis BL. Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 3 virus, Bangkok genotype. The Southeast Asian Journal of Tropical Medicine and Public Health. 1998 Jun; 29(2): 361-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35781
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/1998-29-2/1998-29-2-361.pdfen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshConserved Sequenceen_US
dc.subject.meshDNA, Complementary --chemistryen_US
dc.subject.meshDNA, Viral --chemistryen_US
dc.subject.meshDengue --geneticsen_US
dc.subject.meshDengue Virus --chemistryen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRNA, Viral --geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshTemplates, Geneticen_US
dc.subject.meshThailanden_US
dc.subject.meshViral Nonstructural Proteins --chemistryen_US
dc.titleNucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 3 virus, Bangkok genotype.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
tsajtmph_1998v29n2p361.pdf
Size:
120.35 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Southeast Asian Journal of Tropical Medicine and Public Health