TLC-spectrophometric separation and trace determination of monocrotophos and dichlorvos in enviromental and biological samples.

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dc.contributor.authorJanghel, Etesh Ken_US
dc.contributor.authorRai, J Ken_US
dc.contributor.authorKhan, Sen_US
dc.contributor.authorRai, M Ken_US
dc.contributor.authorGupta, V Ken_US
dc.date.accessioned2007-04-15en_US
dc.date.accessioned2009-06-02T05:13:50Z
dc.date.available2007-04-15en_US
dc.date.available2009-06-02T05:13:50Z
dc.date.issued2007-04-15en_US
dc.description.abstractOrganophosphorus insecticides, monocrotophos and dichlrovos are increasingly being used in agriculture to control insects on a wide range of crops. Their ready access has resulted in misuse in many instances of homicidal and suicidal poisoning cases. This paper describes about a chromogenic spray reagent for the detection/determination of monocrophos and dichlrovos in environmental and biological samples by TLC and spectrophotometric method. Monocrotophos and dichlorvos on alkaline hydrolysis yield N-methyl acetoacetamide and dichlroacetaldehyde respectively, which in turn react with diazotized p-amino acetophenone to give red-violet and red coloured compounds. Other organophosphorus insecticides do not give this reaction. Moreover, organochlorine and synthetic pyrethroid insecticides and constituents of viscera (amino acids, peptides, proteins etc), which are generally coextracted with the insecticides, do not interfere. However, phenolic compounds and hydrolysed product of carbamate insecticides may interfere and differentiate from monocrotophos and dichlrovos by Rf values. The lower limit of detection is 0.2 mg for monocrotophos and 0.1 mg for dichlorovos. The absorption maxima of the reddish-violet and red colour formed by monocrotophos and dichlrovos, are measured at 560 nm and 540 nm respectively. Beer's Law is obeyed over the concentration range of 1.2 to 6.8 mg and 6.2 to 35 mg in the final solution volume of 25 mL. The molar absorptivity and Sandell's sensitivity of monocrotophos and dichlrovos were found to be 7.1 x 10(5) (+100) 1 mole(-1) cm(-1) and 0.008 mg cm(-2), 1.2 x 10(5) 1 mole(-1) cm(-1) and 0.003 mg cm(-2) respectively. The standard deviation and relative standard deviation were found be +/- 0.005 and 2.05% +/- 0.007 and 2.02% respectively. The developed method has been successfully applied to the detection and determination of monocrotophos and dichlrovos in environmental and biological samples.en_US
dc.description.affiliationChhattisgarh Council of Science & Technology, Pt. Ravishankar Shukla University Campus Raipur, Chhattisgarh, India.en_US
dc.identifier.citationJanghel EK, Rai JK, Khan S, Rai MK, Gupta VK. TLC-spectrophometric separation and trace determination of monocrotophos and dichlorvos in enviromental and biological samples. Journal of Environmental Science & Engineering. 2007 Apr; 49(2): 133-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/114099
dc.language.isoengen_US
dc.source.urihttps://www.neeri.res.in/jese.htmlen_US
dc.subject.meshChemistry, Analytical --methodsen_US
dc.subject.meshChromatography, Thin Layer --methodsen_US
dc.subject.meshDichlorvos --analysisen_US
dc.subject.meshEnvironmental Monitoring --methodsen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIndicators and Reagents --pharmacologyen_US
dc.subject.meshMonocrotophos --analysisen_US
dc.subject.meshPesticide Residues --analysisen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSolvents --analysisen_US
dc.subject.meshSpectrophotometry --methodsen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshWater Pollutants, Chemical --analysisen_US
dc.titleTLC-spectrophometric separation and trace determination of monocrotophos and dichlorvos in enviromental and biological samples.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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