Programmed cell death as a prognostic indicator for radiation therapy in cervical carcinoma patients: a pilot study.

Simple item page
dc.contributor.authorBhosle, S Men_US
dc.contributor.authorHuilgol, N Gen_US
dc.contributor.authorMishra, K Pen_US
dc.date.accessioned2005-01-14en_US
dc.date.accessioned2009-06-01T16:27:25Z
dc.date.available2005-01-14en_US
dc.date.available2009-06-01T16:27:25Z
dc.date.issued2005-01-14en_US
dc.description.abstractPURPOSE: In clinical practice, radiation therapy often fails in cervical carcinoma stage IIIB and there is a need to develop a predictive assay for prognosis of radiation treatment outcome in cancer patient. We have attempted to evaluate the relevance of changes in Membrane Fluidity (MF) and associated apoptotic cell death in cervical cancer cells after first fractionated dose of radiation therapy to treatment outcome of stage IIIB cervical carcinoma patients. MATERIALS AND METHODS: Biopsies of 15 patients with histologically proven cervix cancer were collected from the patients before and 24 h after first fractionated radiation dose of 2 grays (Gy). Cell suspension made in Dulbecco's Modified Eagle's Medium (DMEM) were used for further investigations and cell suspension of cervix cancer patient were used to measure MF by fluorescence polarization method and apoptotic index (AI) was determined by Tdt dUTP Nucleotide End Labeling (TUNEL) assay. RESULTS: A substantial increase in MF and AI was observed in cervical cancer cells irradiated ex vivo . A significant correlation ( P P > 0.1) was detected between changes in MF and treatment outcome of patients. CONCLUSION: Preliminary results showed significant change in MF and a marked increase in percentage apoptosis of cervix cancer cells irradiated ex vivo . The changes in AI after first fractionated dose of radiotherapy in cervical carcinoma patients may provide a predictor of prognosis for radiotherapy in uterine cervical carcinoma patients.en_US
dc.description.affiliationRadiation Oncology Division, Dr. Balabhai Nanavati Hospital, Mumbai, India.en_US
dc.identifier.citationBhosle SM, Huilgol NG, Mishra KP. Programmed cell death as a prognostic indicator for radiation therapy in cervical carcinoma patients: a pilot study. Journal of Cancer Research and Therapeutics. 2005 Jan-Mar; 1(1): 41-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111565
dc.language.isoengen_US
dc.source.urihttps://www.cancerjournal.neten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshApoptosisen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Nick-End Labelingen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPilot Projectsen_US
dc.subject.meshPrognosisen_US
dc.subject.meshTreatment Outcomeen_US
dc.subject.meshUterine Cervical Neoplasms --pathologyen_US
dc.titleProgrammed cell death as a prognostic indicator for radiation therapy in cervical carcinoma patients: a pilot study.en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Cancer Research and Therapeutics