Use of reverse transcription polymerase chain reaction for the detection of Mycobacterium leprae in the slit-skin smears of leprosy patients.

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dc.contributor.authorJadhav, R Sen_US
dc.contributor.authorKamble, R Ren_US
dc.contributor.authorShinde, V Sen_US
dc.contributor.authorEdward, Sen_US
dc.contributor.authorEdward, V Ken_US
dc.date.accessioned2005-04-28en_US
dc.date.accessioned2009-05-28T10:05:23Z
dc.date.available2005-04-28en_US
dc.date.available2009-05-28T10:05:23Z
dc.date.issued2005-04-28en_US
dc.description.abstractThe relevance of bacterial index (BI) for understanding the prognosis of leprosy patients on treatment has been extensively debated, as it does not give a very clear idea of the viability of the bacteria in patients under treatment. Here we used slit-skin smear samples of leprosy patients to test the suitability for studying viability of Mycobacterium leprae using reverse transcription polymerase chain reaction (RT-PCR). For this purpose, we recruited 13 multibacillary (MB) leprosy patients (8 lepromatous and 5 borderline lepromatous). Of these, 7 were relapse cases, 3 were under treatment (MB-MDT), 2 were new cases and 1 had completed treatment. We carried out extraction of RNA using Trizol reagent (Life Technologies, UK) from the slit-skin smear samples from these patients. The RNA preparation was then used for the RT-PCR using Mycobacterium leprae-specific primers for the fragment of 16s ribosomal RNA gene. Samples from both the new cases, 4 suspected relapse cases and 1 patient under treatment showed positive RT-PCR results. Other 6 patients whose smear samples did not show any amplification by RT-PCR were on MB-MDT from 8 to 30 months. The usefulness of the technique needs to be validated using mouse footpad technique and also should be more extensively explored for studying the viability of M. leprae, the efficacy of treatment and the presence of other mycobacteria in the slit-skin smear samples.en_US
dc.description.affiliationStanley Browne Laboratory, Richardson Leprosy Hospital Sangli-Miraj Road, Miraj 416 410, Maharashtra, India. rupenjadhav@yahoo.comen_US
dc.identifier.citationJadhav RS, Kamble RR, Shinde VS, Edward S, Edward VK. Use of reverse transcription polymerase chain reaction for the detection of Mycobacterium leprae in the slit-skin smears of leprosy patients. Indian Journal of Leprosy. 2005 Apr-Jun; 77(2): 116-27en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/54832
dc.language.isoengen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshElectrophoresis, Agar Gelen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLeprosy, Borderline --microbiologyen_US
dc.subject.meshLeprosy, Lepromatous --microbiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMycobacterium leprae --geneticsen_US
dc.subject.meshRNA, Bacterial --chemistryen_US
dc.subject.meshRNA, Ribosomal, 16S --chemistryen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleUse of reverse transcription polymerase chain reaction for the detection of Mycobacterium leprae in the slit-skin smears of leprosy patients.en_US
dc.typeJournal Articleen_US
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