Diagnosis of pulmonary tuberculosis by polymerase chain reaction for MPB64 gene: an evaluation in a blind study.

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dc.contributor.authorDar, Len_US
dc.contributor.authorSharma, S Ken_US
dc.contributor.authorBhanu, N Ven_US
dc.contributor.authorBroor, Sen_US
dc.contributor.authorChakraborty, Men_US
dc.contributor.authorPande, J Nen_US
dc.contributor.authorSeth, Pen_US
dc.date.accessioned1998-01-04en_US
dc.date.accessioned2009-05-27T10:09:38Z
dc.date.available1998-01-04en_US
dc.date.available2009-05-27T10:09:38Z
dc.date.issued1998-01-04en_US
dc.description.abstractPolymerase chain reaction (PCR) has been found to be a sensitive and rapid method to confirm a clinical diagnosis of tuberculosis. We evaluated PCR for M. tuberculosis complex specific MPB64 gene for the diagnosis of pulmonary tuberculosis, in a double blind study. One hundred and eighty-two clinical samples (sputum, bronchioalveolar lavage and pleural fluid) from patients with a clinical diagnosis of pulmonary tuberculosis and 72 samples from patients with non-tubercular pulmonary lesions and normal healthy individuals were included. The samples were coded and clinical details were concealed from the laboratory, where conventional diagnostic methods and PCR were carried out independent of each other. On decoding and analysing the data, PCR was positive in 59% of single sputum samples from clinically diagnosed pulmonary tuberculosis, while M. tuberculosis could be grown in 18% of the samples. PCR could identify M. tuberculosis in 81.8% of the culture positive sputum samples. PCR was also positive in 71.4% of bronchioalveolar lavage (BAL) fluid and 60.7% pleural fluid samples from clinically suspected cases, which were mostly culture negative. On comparison with response to treatment, PCR was positive in 79.5% of patients who improved on anti-tuberculosis treatment, with a positive predictive value of 92%. PCR for MPB64 gene provides a useful alternative for the diagnosis of pulmonary tuberculosis from sputum and paucibacillary samples like BAL and pleural fluid in which conventional methods show low sensitivity, especially in areas from which strains show a low copy number of other PCR targets like the IS 6110 insertion sequence.en_US
dc.description.affiliationDepartment of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.en_US
dc.identifier.citationDar L, Sharma SK, Bhanu NV, Broor S, Chakraborty M, Pande JN, Seth P. Diagnosis of pulmonary tuberculosis by polymerase chain reaction for MPB64 gene: an evaluation in a blind study. The Indian Journal of Chest Diseases & Allied Sciences. 1998 Jan-Mar; 40(1): 5-16en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/29230
dc.language.isoengen_US
dc.source.urihttps://medind.nic.in/iae/iaem.shtmlen_US
dc.subject.meshBronchoalveolar Lavage Fluid --microbiologyen_US
dc.subject.meshDNA, Bacterial --analysisen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshHumansen_US
dc.subject.meshMycobacterium tuberculosis --geneticsen_US
dc.subject.meshPleural Effusion --microbiologyen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSputum --microbiologyen_US
dc.subject.meshTuberculosis, Pulmonary --diagnosisen_US
dc.titleDiagnosis of pulmonary tuberculosis by polymerase chain reaction for MPB64 gene: an evaluation in a blind study.en_US
dc.typeClinical Trialen_US
dc.typeComparative Studyen_US
dc.typeControlled Clinical Trialen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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