Report of rpoB mutation in clinically suspected cases of drug resistant leprosy: A study from Eastern India.

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dc.contributor.authorReja, Abu Hena Hasanoor
dc.contributor.authorBiswas, Nibir
dc.contributor.authorBiswas, Supratik
dc.contributor.authorLavania, Mallika
dc.contributor.authorChaitanya, Vedithi Sundeep
dc.contributor.authorBanerjee, Surajita
dc.contributor.authorPatra, Prasanta Sinha Maha
dc.contributor.authorGupta, Umesh Dutta
dc.contributor.authorPatra, Pradip Kumar
dc.contributor.authorSengupta, Utpal
dc.contributor.authorBhattacharya, Basudev
dc.date.accessioned2015-06-24T06:24:50Z
dc.date.available2015-06-24T06:24:50Z
dc.date.issued2015-03
dc.description.abstractBackground: The current strategy for leprosy control depends mainly on early case detection and providing the recommended multidrug therapy (MDT) dosage. Understanding the molecular mechanisms of drug resistance to each of these drugs is essential in providing effective treatment and preventing the spread of resistant strains in the community. The progress of molecular biology research provides a very effi cient opportunity for the diagnosis of drug resistance by in vitro method. Aim: We aimed to investigate the point mutations within the rpoB gene region of the Mycobacterium leprae genome, which are responsible for resistance to rifampicin, in order to determine the emergence of drug resistance in leprosy in the Kolkata region of West Bengal. Methods: A total of 50 patients with a relapse of leprosy were enrolled in the study. Skin smears were obtained for estimation of bacillary index and biopsies were obtained in 70% alcohol for extraction of DNA. The extracted DNA was amplifi ed by M. leprae-polymerase chain reaction (PCR) targeting rpoB gene region. Every single nucleotide base in the sequence is aligned to reference sequence and identity gaps were determined by NCBI – BLAST. Later in-silico analysis was done to identify the changes in the translated protein sequences. Results: A mutation at the base pair position 2275405 where G is replaced by C in the M. leprae genome, which corresponds to the coding region of rpoB gene (279 bp – 2275228 to2275506), was observed in two patients. This missense mutation in CAC codon brings about a glutamic acid to histidine change in the amino acid sequence of RNA polymerase beta subunit at the position 442 (Glu442His), a region specifi c for rifampicin interaction, which might be responsible for unresponsiveness to rifampicin by manifesting a stable bacteriological index in these 2 patients even after completion of 24 months of multibacillary multi-drug therapy (MB-MDT). Limitations: The major limitations of multipleprimer PCR amplifi cation refractory mutation system (MARS) assay is that it capable of detecting mutation at codon 425 and cannot distinguish any silent amino acid changes. Conclusion: The study indicates the existence of rifampicin drug resistance in Eastern India.en_US
dc.identifier.citationReja Abu Hena Hasanoor, Biswas Nibir, Biswas Supratik, Lavania Mallika, Chaitanya Vedithi Sundeep, Banerjee Surajita, Maha Patra Prasanta Sinha, Gupta Umesh Dutta, Patra Pradip Kumar, Sengupta Utpal, Bhattacharya Basudev. Report of rpoB mutation in clinically suspected cases of drug resistant leprosy: A study from Eastern India. Indian Journal of Dermatology, Venereology and Leprology. 2015 Mar-Apr; 81(2): 155-161.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158267
dc.language.isoenen_US
dc.source.urihttps://www.ijdvl.com/article.asp?issn=0378-6323;year=2015;volume=81;issue=2;spage=155;epage=161;aulast=Hasanooren_US
dc.subjectDNA Sequencingen_US
dc.subjectdrug resistanceen_US
dc.subjectpoint mutationsen_US
dc.subjectrifampicinen_US
dc.subjectRNA polymerase beta subuniten_US
dc.subject.meshBacterial Proteins --genetics
dc.subject.meshDNA-Directed RNA Polymerases --genetics
dc.subject.meshDrug Resistance --genetics
dc.subject.meshHumans
dc.subject.meshIndia
dc.subject.meshLeprosy --drug therapy
dc.subject.meshMutation
dc.subject.meshRifampin
dc.subject.meshSequence Analysis, DNA --methods
dc.titleReport of rpoB mutation in clinically suspected cases of drug resistant leprosy: A study from Eastern India.en_US
dc.typeArticleen_US
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