Flow cytometric crossmatch for kidney transplantation.

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dc.contributor.authorKoktathong, Krisadaen_US
dc.contributor.authorVejbaesya, Sasijiten_US
dc.contributor.authorBejrachandra, Sastornen_US
dc.contributor.authorPattanapanyasat, Koviten_US
dc.date.accessioned2009-05-27T21:57:54Z
dc.date.available2009-05-27T21:57:54Z
dc.date.issued2005-06-09en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractSerum samples from 49 patients with panel reactive antibodies of greater than 15% and 17 patients who have related donor pairs were collected at the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Crossmatching was performed by three methods, flow cytometry crossmatch (FCXM), the standard National Institutes of Health (NIH), and the antihuman globulin (AHG) microlymphocytotoxicity. 28.9% Spell out of both T- and B-cell crossmatch was positive by FCXM and negative by NIH and AHG. When the T-cell and B-cell crossmatches were negative by FCXM, they were negative by both NIH- and AHG method. There was significant difference of the crossmatch result between FCXM and NIH and between FCXM and AHG (p < 0.0001). In addition, FCXM was about 4-16 and 8-32 times more sensitive than AHG- and NIH method, respectively. In conclusion, the result of FCXM is clear and this method is more sensitive than NIH- and AHG method FCXM should be used together with the NIH- and AHG method for kidney transplantation.en_US
dc.description.affiliationDepartment of the Transfusion Medicine, Faculty of Medicine, Siriraj Hospital, Bangkok 10700, Thailand.en_US
dc.identifier.citationKoktathong K, Vejbaesya S, Bejrachandra S, Pattanapanyasat K. Flow cytometric crossmatch for kidney transplantation. Journal of the Medical Association of Thailand. 2005 Jun; 88(6): 769-74en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/45711
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshB-Lymphocytes --immunologyen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHistocompatibility Testingen_US
dc.subject.meshHumansen_US
dc.subject.meshKidney Transplantation --immunologyen_US
dc.subject.meshT-Lymphocytes --immunologyen_US
dc.subject.meshTissue Donorsen_US
dc.titleFlow cytometric crossmatch for kidney transplantation.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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