Glucose & sodium chloride induced biofilm production & ica operon in clinical isolates of staphylococci.

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dc.contributor.authorAgarwal, Astha
dc.contributor.authorJain, Amita
dc.date.accessioned2013-12-02T05:12:13Z
dc.date.available2013-12-02T05:12:13Z
dc.date.issued2013-08
dc.description.abstractBackground & objectives: All colonizing and invasive staphylococcal isolates may not produce biofilm but may turn biofilm producers in certain situations due to change in environmental factors. This study was done to test the hypothesis that non biofilm producing clinical staphylococci isolates turn biofilm producers in presence of sodium chloride (isotonic) and high concentration of glucose, irrespective of presence or absence of ica operon. Methods: Clinical isolates of 100 invasive, 50 colonizing and 50 commensal staphylococci were tested for biofilm production by microtiter plate method in different culture media (trypticase soy broth alone or supplemented with 0.9% NaCl/ 5 or 10% glucose). All isolates were tested for the presence of ica ADBC genes by PCR. Results: Biofilm production significantly increased in the presence of glucose and saline, most, when both glucose and saline were used together. All the ica positive staphylococcal isolates and some ica negative isolates turned biofilm producer in at least one of the tested culture conditions. Those remained biofilm negative in different culture conditions were all ica negative. Interpretation & conclusions: The present results showed that the use of glucose or NaCl or combination of both enhanced biofilm producing capacity of staphylococcal isolates irrespective of presence or absence of ica operon.en_US
dc.identifier.citationAgarwal Astha, Jain Amita. Glucose & sodium chloride induced biofilm production & ica operon in clinical isolates of staphylococci. Indian Journal of Medical Research. 2013 Aug; 138(2): 262-266.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/149490
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788214/?report=classicen_US
dc.subjectBiofilm productionen_US
dc.subjectglucoseen_US
dc.subjectica operonen_US
dc.subjectNaClen_US
dc.subjectstaphylococcien_US
dc.titleGlucose & sodium chloride induced biofilm production & ica operon in clinical isolates of staphylococci.en_US
dc.typeArticleen_US
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