Rapid purification of secretory acid proteinase from Candida albicans and its characterization.

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dc.contributor.authorBanerjee, Aen_US
dc.contributor.authorGanesan, Ken_US
dc.contributor.authorDatta, Aen_US
dc.date.accessioned1991-10-01en_US
dc.date.accessioned2009-05-27T09:42:57Z
dc.date.available1991-10-01en_US
dc.date.available2009-05-27T09:42:57Z
dc.date.issued1991-10-01en_US
dc.description.abstractSecretory acid proteinase from C. albicans was purified from culture supernatant to apparent homogeneity by ion-exchange chromatography. Two isozymes of the proteinase were resolved using a novel chromatofocusing method. The enzyme, which appears to be a glycoprotein, consists of a single polypeptide chain with glutamine at the N-terminus. Its molecular weight is about 45,000 and isoelectric point is pH 4.6. At pH 5, the proteinase is stable at 45 degrees C for at least 15 min. It has a broad substrate specificity. With BSA as a substrate, Km was determined to be 1.6 x 10(-4) M. The enzyme is inhibited by pepstatin and thus is a carboxyl proteinase. It undergoes autocatalytic digestion at or below pH 5.0. The kinetics of induction of proteinase by various proteins are also reported.en_US
dc.description.affiliationMolecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi.en_US
dc.identifier.citationBanerjee A, Ganesan K, Datta A. Rapid purification of secretory acid proteinase from Candida albicans and its characterization. Indian Journal of Biochemistry & Biophysics. 1991 Oct-Dec; 28(5-6): 444-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28344
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAmino Acids --analysisen_US
dc.subject.meshCandida albicans --enzymologyen_US
dc.subject.meshEndopeptidases --isolation & purificationen_US
dc.subject.meshIsoelectric Pointen_US
dc.subject.meshIsoenzymes --isolation & purificationen_US
dc.subject.meshKineticsen_US
dc.subject.meshMolecular Weighten_US
dc.titleRapid purification of secretory acid proteinase from Candida albicans and its characterization.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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