Effect of cholesterol on multiplication, lipid metabolism and lysosomal enzymes of Hartmanella culbertsoni.
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Date
1979-06
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Abstract
Addition of sonicated dispersions of cholesterol to peptone-salt-vitamin
medium resulted in the metabolism of the sterol by Hartmanella culbertsoni. Trophozoite
multiplication was stimulated at 1–5 mg/litre, but retarded at 10-20 mg/litre.
When cholesterol was added to the medium, incorporation of [1,2—14C] –acetate
into neutral lipid, phospholipid, non-saponifiable and cholesterol fractions of the
amoebae was significantly reduced. Cholesterol ester was detected in the medium
but phospholipids were not released. Addition of cholesterol stimulated the
activity of lysosomal acid phosphatase, acid deoxyribonuclease and cathepsin Β
but did not affect 5'-nucleotidase, adenosine triphosphatase, alkaline phosphatase,
glucose-6-phosphatase, succinate dehydrogenase and cytochrome C oxidase.
Description
Keywords
Cholesterol, trophozoite-multiplication, phospholipid, lysosomal enzymes
Citation
Lal A A, Garg N K. Effect of cholesterol on multiplication, lipid metabolism and lysosomal enzymes of Hartmanella culbertsoni. Journal of Biosciences. 1979 Jun; 1(2): 151-157.