Expansion of peripheral and intratumoral regulatory T-cells in hepatocellular carcinoma: A case-control study.

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dc.contributor.authorThakur, Sapna
dc.contributor.authorSingla, Anuj
dc.contributor.authorChawla, Yogesh
dc.contributor.authorRajwanshi, Arvind
dc.contributor.authorKalra, Naveen
dc.contributor.authorArora, Sunil K
dc.date.accessioned2012-10-11T05:08:55Z
dc.date.available2012-10-11T05:08:55Z
dc.date.issued2011-07
dc.description.abstractBackground: Hepatocellular carcinoma (HCC) is notorious for poor prognosis with limited therapeutic options. A better understanding of the role of regulatory T-cells (Tregs) in HCC is important for design of immunotherapy based clinical protocol. The objective of the present study was to evaluate the presence of Tregs in tumor microenvironment in patients with HCC compared to chronic hepatitis (CH). Materials and Methods: The frequency of CD4 + CD25 + Treg cells was evaluated from peripheral blood (PB) of 28 patients of HCC and 30 controls including CH cases and healthy donors using flowcytometry. Intratumoral Treg were also analyzed in tissue samples from 17 HCC cases and 15 CH cases. In addition the expression of FOXP3 and CTLA-4 was also studied by RT-PCR. Results: Frequency of CD4 + CD25 + cells in the PBMCs of HCC cases was significantly higher than in HC (10.8 ± 7.64 vs 3.05 ± 1.30, P < 0.005) and CH patients (2.88 ± 1.92, P < 0.005). Also Treg population was significantly higher in HCC tumor microenvironment compared to CH biopsies (15.8 ± 5.32 vs 5.51 ± 3.40, P < 0.05). Expression of FOXP3 and CTLA-4 was also significantly higher in HCC patients ( P < 0.05) compared to CH group. Conclusions: We provide evidence of an increased population of Treg not only in the PB but also in tumor microenvironment of HCC patients, suggesting association of enhanced Treg activity with poor immune responses to tumor antigens. These findings may in future play a significant role in designing immunotherapeutic approaches in HCC.en_US
dc.identifier.citationThakur Sapna, Singla Anuj, Chawla Yogesh, Rajwanshi Arvind, Kalra Naveen, Arora Sunil K. Expansion of peripheral and intratumoral regulatory T-cells in hepatocellular carcinoma: A case-control study. Indian Journal of Pathology & Microbiology. 2011 Jul-Sept 54(3): 448-453.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/142023
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2011;volume=54;issue=3;spage=448;epage=453;aulast=Thakuren_US
dc.subjectCTLA-4en_US
dc.subjectFOXP3en_US
dc.subjecthepatocellular carcinomaen_US
dc.subjectimmuneregulationen_US
dc.subjectT-regulatory cellsen_US
dc.subject.meshAdult
dc.subject.meshAntigens, CD4 --analysis
dc.subject.meshCTLA-4 Antigen --analysis
dc.subject.meshCarcinoma, Hepatocellular --immunology
dc.subject.meshCarcinoma, Hepatocellular --pathology
dc.subject.meshCase-Control Studies
dc.subject.meshFemale
dc.subject.meshFlow Cytometry
dc.subject.meshForkhead Transcription Factors --analysis
dc.subject.meshHumans
dc.subject.meshInterleukin-2 Receptor alpha Subunit --analysis
dc.subject.meshLiver --pathology
dc.subject.meshLiver Neoplasms --immunology
dc.subject.meshLiver Neoplasms --pathology
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshT-Lymphocytes, Regulatory --chemistry
dc.subject.meshT-Lymphocytes, Regulatory --immunology
dc.titleExpansion of peripheral and intratumoral regulatory T-cells in hepatocellular carcinoma: A case-control study.en_US
dc.typeArticleen_US
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