Plasminogen activator: Isolation and purification from lymphosarcoma of ascites bearing mice.
| dc.contributor.author | Nulkar, M W | |
| dc.contributor.author | Darad, Rukmini | |
| dc.contributor.author | Subramanian, M | |
| dc.contributor.author | Pawse, A R | |
| dc.date.accessioned | 2015-07-28T11:22:18Z | |
| dc.date.available | 2015-07-28T11:22:18Z | |
| dc.date.issued | 1991-12 | |
| dc.description.abstract | Plasminogen activator secreted by lymphosarcoma (ascites) of mice was purified up to 163-fold by ammonium sulphate fractionation at 35% saturation and chromatography on p-aminobenzamidine-Sepharose 4B. The purified activator contained specific activity of 9980 IU/mg. The plasminogen activator displayed homogeneity by polyacrylamide slab gel electrophoresis and high performance liquid chromatography. The activator consisted of a single polypeptide chain with an apparent molecular weight of 66,000 daltons as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis under reducing conditions as well as gel filtration on Sephadex G-100. Distinct differences between this activator and urokinase were discernible in respect of specific activities, fibrin affinity and immunochemical properties. The lymphosarcoma activator appears to be of tissue-type origin since it showed gross similarity to standard tissue plasminogen activator in terms of modes of binding to fibrin and immunological attributes. | en_US |
| dc.identifier.citation | Nulkar M W, Darad Rukmini, Subramanian M, Pawse A R. Plasminogen activator: Isolation and purification from lymphosarcoma of ascites bearing mice. Journal of Biosciences. 1991 Dec; 16(4): 223-233. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/160800 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ias.ac.in/jarch/jbiosci/16/223-233.pdf | en_US |
| dc.subject | Plasminogen activator | en_US |
| dc.subject | urokinase | en_US |
| dc.subject | fibrinolysis | en_US |
| dc.subject | Fibrin | en_US |
| dc.subject | lymphosarcoma | en_US |
| dc.title | Plasminogen activator: Isolation and purification from lymphosarcoma of ascites bearing mice. | en_US |
| dc.type | Article | en_US |