Immunoreactive insulin and insulin degrading enzymes in erythrocytes. A preliminary report.

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dc.contributor.authorSnehalatha, Cen_US
dc.contributor.authorTimothy, Hen_US
dc.contributor.authorMohan, Ven_US
dc.contributor.authorRamachandran, Aen_US
dc.contributor.authorViswanathan, Men_US
dc.date.accessioned1990-08-01en_US
dc.date.accessioned2009-05-31T04:19:40Z
dc.date.available1990-08-01en_US
dc.date.available2009-05-31T04:19:40Z
dc.date.issued1990-08-01en_US
dc.description.abstractImmunoreactive insulin (IRI) and insulin degrading enzyme activity (IDEA) of the plasma and the corresponding erythrocyte lysate were estimated in 21 normal volunteers, 18 non insulin dependent diabetic patients (NIDDM), and 16 insulin dependent diabetics (IDDM). The erythrocytes contained several-fold higher concentrations of IRI than in plasma, both in normal and diabetic subjects. The values in controls ranged from 80 to 458 uU/ml against a range of 5 to 25 uU/ml in the corresponding plasma samples. The IRI contents of the diabetic patients were also similar. It showed no correlation to the fasting plasma glucose or the plasma IRI. Following an oral glucose load, no change occurred in the IRI content of the erythrocytes, unlike the changes seen in plasma. The IRI content of the lysate increased with dilution of the sample. The IDEA was higher in diabetic patients compared to controls, especially so in the IDDM (P less than 0.01). It also showed more than one peak activity at different pH of the reaction buffer, indicating the possibility of a complex of enzymes. Human erythrocytes contain large pools of IRI and its degrading enzymes. The significance of the pool of the insulin in non-target tissue needs to be studied.en_US
dc.description.affiliationDiabetes Research Centre, Madras.en_US
dc.identifier.citationSnehalatha C, Timothy H, Mohan V, Ramachandran A, Viswanathan M. Immunoreactive insulin and insulin degrading enzymes in erythrocytes. A preliminary report. Journal of the Association of Physicians of India. 1990 Aug; 38(8): 558-61en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/94658
dc.language.isoengen_US
dc.source.urihttps://www.japi.orgen_US
dc.subject.meshAdulten_US
dc.subject.meshBlood Glucose --analysisen_US
dc.subject.meshDiabetes Mellitus, Type 1 --blooden_US
dc.subject.meshDiabetes Mellitus, Type 2 --blooden_US
dc.subject.meshErythrocytes --enzymologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInsulin --blooden_US
dc.subject.meshMiddle Ageden_US
dc.titleImmunoreactive insulin and insulin degrading enzymes in erythrocytes. A preliminary report.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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