Current Microbial Isolates from Wound Swab and Their Susceptibility Pattern in a Private Medical College Hospital in Dhaka city.
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Date
2015-01
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Abstract
Background: Wound infection is one of the major health problems that are caused and
aggravated by the invasion of pathogenic organisms where empiric treatment is routine.
Objective: To isolate and identify the bacteria causing wound infection and to
determine the antimicrobial susceptibility pattern. Materials and method: A total of 263
wound swab and pus samples were collected during the period of January to December
2012 from Delta Medical College and Hospital, Dhaka, Bangladesh. Swabs from the
wound were inoculated on appropriate media and cultured and the isolates were
identified by standard procedures as needed. Antimicrobial susceptibility testing was
performed by disk diffusion method according to ‘The Clinical Laboratory Standard
Institute’ guidelines. Results: In this study 220 bacterial isolates were recovered from
263 samples showing an isolation rate of 83.65%. The predominant bacteria isolated
from infected wounds were Staphylococcus aureus 89 (40.45%) followed by Escherichia
coli 62 (28.18%), Pseudomonas aeruginosa 34 (15.45%), Enterococci 18 (8.18%),
Acinetobacter 5 (2.27%), Klebsiella 9 (4.09%) and Proteus 3 (3.36%). Staphylococcus
aureus was sensitive to linezolid (94.38%), fusidic acid (91.01%), vancomycin
(87.64%), amikacin (74.15%) and gentamicin (73.03%). Among the Gram negative
isolates Escherichia coli was predominant and showed sensitivity to imipenem
(93.54%) amikacin (83.87%) colistin (53.22%) and piperacillin and tazobactum
(53.22%) and pseudomonas showed sensitivity to amikacin (73.52%), imipenem
(70.58%) and colistin (70.58%). Conclusion: Staphylococcus aureus was the most
frequently isolated pathogen from wound swab and the antibiotic sensitivity pattern of
various isolates help to assist the clinician in appropriate selection of empirical
antibiotics against wound infection.
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Keywords
Wound swab, isolation of bacteria, antimicrobial sensitivity pattern
Citation
Sultana Shahin, Mawla Nurunnahar, Kawser Shamima, Akhtar Nayareen, Ali Md Khoybar. Current Microbial Isolates from Wound Swab and Their Susceptibility Pattern in a Private Medical College Hospital in Dhaka city. Delta Medical College Journal. 2015 Jan; 3(1): 25-30.