A high yield DNA extraction method for medically important Candida species: A comparison of manual versus QIAcube‑based automated system.

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dc.contributor.authorDas, P
dc.contributor.authorPandey, P
dc.contributor.authorHarishankar, A
dc.contributor.authorChandy, M
dc.contributor.authorBhattacharya, S
dc.date.accessioned2017-01-04T12:17:55Z
dc.date.available2017-01-04T12:17:55Z
dc.date.issued2016-10
dc.description.abstractThe prognosis of infected individuals with candidemia depends on rapid and precise diagnosis which enables optimising treatment. Three fungal DNA extraction protocols have been compared in this study for medically important Candida species. The quality and quantity of the DNA extracted by physical, chemical and automated protocols was compared using NanoDrop ND-2000 spectrophotometer. It was found that the yield and purity (260/230) ratio of extracted DNA was significantly high in the physical treatment‑based protocol as compared to chemical based or automated protocol. Extracted DNA‑based real time‑polymerase chain reaction showed an analytical sensitivity of 103 cfu/mL. The result of this study suggests physical treatment is the most successful extraction technique compared to other two protocols.en_US
dc.identifier.citationDas P, Pandey P, Harishankar A, Chandy M, Bhattacharya S. A high yield DNA extraction method for medically important Candida species: A comparison of manual versus QIAcube‑based automated system. Indian Journal of Medical Microbiology. 2016 Oct-Dec; 34(4): 533-535.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/181125
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2016;volume=34;issue=4;spage=533;epage=535;aulast=Dasen_US
dc.subjectAutomated extractionen_US
dc.subjectCandida spp.en_US
dc.subjectDNA extractionen_US
dc.subjectmanual extractionen_US
dc.subjectreal‑time polymerase chain reactionen_US
dc.titleA high yield DNA extraction method for medically important Candida species: A comparison of manual versus QIAcube‑based automated system.en_US
dc.typeArticleen_US
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