A four-element based transposon system for allele specific tagging in plants--theoretical considerations.

Simple item page
dc.contributor.authorPhogat, Sen_US
dc.contributor.authorBurma, P Ken_US
dc.contributor.authorPental, Den_US
dc.date.accessioned2000-03-29en_US
dc.date.accessioned2009-06-01T14:43:35Z
dc.date.available2000-03-29en_US
dc.date.available2009-06-01T14:43:35Z
dc.date.issued2000-03-29en_US
dc.description.abstractThe two-element transposon constructs, utilizing either Ac/Ds or Spm/dSpm, allow random tagging of genes in heterologous model species, but are inadequate for directed tagging of specific alleles of agronomic importance. We propose the use of Ac/Ds in conjunction with Spm/dSpm to develop a four-element system for directed tagging of crop-specific alleles. The four-element based construct would include both Ds and dSpm along with relevant marker genes and would function in two steps. In the first step dSpm(Ds) stocks (a minimum of two) would be crossed to a line containing transposases of Spm and unlinked integrations would be selected from segregating population by the use of a negative selection marker to develop stocks representing integration of dSpm(Ds) at a large number of locations in the genome. Selections would be made for a line in which dSpm(Ds) shows partial or complete linkage to the allele of interest. In the second step selected line would be crossed to a line containing Ac transposase to induce transpositions of Ds element to linked sites thereby exploiting the natural tendency of Ds element to jump to linked sites. Unlinked jumps of dSpm(Ds) and linked jumps of Ds could be monitored by appropriate marker genes. The proposed model would allow tagging of allele of interest in chromosome addition lines and also help in the efficient use of genic male sterility systems for hybrid seed production by tightly marking the fertility restorer gene with a negative selection marker.en_US
dc.description.affiliationDepartment of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India.en_US
dc.identifier.citationPhogat S, Burma PK, Pental D. A four-element based transposon system for allele specific tagging in plants--theoretical considerations. Journal of Biosciences. 2000 Mar; 25(1): 57-63en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111032
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAllelesen_US
dc.subject.meshChimera --geneticsen_US
dc.subject.meshDNA Transposable Elementsen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshPlants --geneticsen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshReproductionen_US
dc.subject.meshSeeds --geneticsen_US
dc.titleA four-element based transposon system for allele specific tagging in plants--theoretical considerations.en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Biosciences