Influence of Ca2+ on kinetics and thermodynamics of the NADPH-dependent microsomal lipid peroxidation.

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dc.contributor.authorGupta, Men_US
dc.contributor.authorKale, R Ken_US
dc.date.accessioned1996-10-01en_US
dc.date.accessioned2009-05-27T09:43:05Z
dc.date.available1996-10-01en_US
dc.date.available2009-05-27T09:43:05Z
dc.date.issued1996-10-01en_US
dc.description.abstractThe effect of Ca2+ on kinetics and thermodynamics of lipid peroxidation in microsomes prepared from liver of male Swiss albino mice (7-8 weeks old) was studied. Ca2+ was found to increase the Vmax in temperature dependent manner. Michaelis-Menten constant (Km) also increased with temperature. However, the linearity and extent of change in Km remained unaffected in presence of Ca2+, and was suggestive of non-competitive and mixed type of activation. The activation constant (Ka) obtained by the replotting of slopes of the Lineweaver-Burk plots against the reciprocal of Ca2+ concentration showed linear variation with temperature. The linear pattern of Arrhenius plots indicated non-involvement of parallel reactions of other intermediate species in the lipid peroxidation. Thermodynamic parameters delta H degree, delta S degree and delta G degree, associated with lipid peroxidation process were studied. The positive value of delta H degree is suggestive of the endothermic nature of the process. It appears that the NADPH induced lipid peroxidation is an entropy driven process.en_US
dc.description.affiliationFree Radical Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.en_US
dc.identifier.citationGupta M, Kale RK. Influence of Ca2+ on kinetics and thermodynamics of the NADPH-dependent microsomal lipid peroxidation. Indian Journal of Biochemistry & Biophysics. 1996 Oct; 33(5): 377-83en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28351
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium --pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshLipid Peroxidation --drug effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMicrosomes, Liver --drug effectsen_US
dc.subject.meshNADP --metabolismen_US
dc.subject.meshNADPH-Ferrihemoprotein Reductase --metabolismen_US
dc.subject.meshThermodynamicsen_US
dc.titleInfluence of Ca2+ on kinetics and thermodynamics of the NADPH-dependent microsomal lipid peroxidation.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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