Methyltransferase Like 3 and Fat Mass and Obesity-Associated Protein Are Required for H7N9 Infection in Cell Culture

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dc.contributor.authorLuo, Yingen_US
dc.contributor.authorSun, Yingen_US
dc.contributor.authorLiu, Huien_US
dc.contributor.authorPeng, Boen_US
dc.contributor.authorWu, Weihuaen_US
dc.contributor.authorWang, Xinen_US
dc.contributor.authorZheng, Qingen_US
dc.contributor.authorFang, Shisongen_US
dc.date.accessioned2020-11-18T10:29:12Z
dc.date.available2020-11-18T10:29:12Z
dc.date.issued2020-06
dc.description.abstractBackground METTL3 (methyltransferase like 3) and FTO (fat mass and obesity-associated protein) are criticalfor establishing and regulating the m6A (N6-methyladenosine) modification, but very little is known about thefunction of m6A in the immune system or its role in interactions within the host immune system.Methods Western blotting experiment was used to check the expression of the host proteins related to m6Amodification after H7N9 influenza virus infection. Immunofluorescence experiment was used to identifywhether the subcellular localization of related proteins. Moreover, we knocked down and over expressendogenous METTL3 or FTO in A549 cells, and then infected the cells with H7N9 to test whethermethyltransferases or demethylases affect H7N9 replication.Result We confirmed that the expression pattern of m6A proteins was altered during H7N9 infection. METTL3and FTO were located in the nucleus, and YTHDF3 was located in the cytoplasm. Furthermore, our resultsdemonstrate that downregulation of METTL3 or FTO expression in A549 cells severely impairs viral proteinexpression and H7N9 infection.Conclusions METTL3 and FTO are critical for H7N9 replication which may represent a new mechanism for thecontrol of H7N9 replication and host-pathogen interactions.en_US
dc.identifier.affiliationsCollege of Pharmacy, Jinan University, Guangzhou, Chinaen_US
dc.identifier.affiliationsShenzhen Center for Disease Control and Prevention, Shenzhen, Chinaen_US
dc.identifier.citationLuo Ying, Sun Ying, Liu Hui, Peng Bo, Wu Weihua, Wang Xin, Zheng Qing, Fang Shisong. Methyltransferase Like 3 and Fat Mass and Obesity-Associated Protein Are Required for H7N9 Infection in Cell Culture. Journal of Pharmaceutical and Biomedical Sciences. 2020 Jun; 10(6): 113-118en_US
dc.identifier.issn2230-7885
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215723
dc.languageenen_US
dc.publisherLawarence Press Pvt. Ltd.en_US
dc.relation.issuenumber6en_US
dc.relation.volume10en_US
dc.source.urihttps://lawarencepress.com/ojs/index.php/JPBMS/article/view/614/pdf_298en_US
dc.subjectMETTL3en_US
dc.subjectFTOen_US
dc.subjectH7N9en_US
dc.subjectRNA-seq.en_US
dc.titleMethyltransferase Like 3 and Fat Mass and Obesity-Associated Protein Are Required for H7N9 Infection in Cell Cultureen_US
dc.typeJournal Articleen_US
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