Genomewide analysis of the CIII peroxidase family in sweet orange (Citrus sinensis) and expression profiles induced by Xanthomonas citri subsp. citri and hormones

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dc.contributor.authorLi, Qiangen_US
dc.contributor.authorDou, Wanfuen_US
dc.contributor.authorQi, Jingjingen_US
dc.contributor.authorQin, Xiujuanen_US
dc.contributor.authorChen, Shanchunen_US
dc.contributor.authorHe, Yongruien_US
dc.date.accessioned2020-11-18T10:21:10Z
dc.date.available2020-11-18T10:21:10Z
dc.date.issued2020-01
dc.description.abstractClass III peroxidase (CIII prx) is a plant-specific multigene family that regulates the physiological and stress responses. This research aimed to exhaustively annotate and analyse the CIII prx family in sweet orange and to explore the regulated expression profiles by Xanthomonas citri subsp. citri (Xcc) and plant hormones. We further assessed the relationship between CIII prxs and citrus bacterial canker. The phylogeny, gene structure, conserved motifs, gene duplications and microsynteny of the CIII prx family were analysed. Expression profiles of specific CsPrxs induced by Xanthomonas citri subsp. citri and plant hormones were detected by quantitative reverse transcription-polymerase chain reaction. Subcellular localization was analysed through transient expression assessments. A total of 72 CIII prx members were identified from the genomes of sweet orange. In all chromosomes of sweet orange, the CsPrxs could be detected except in chromosome 8. In addition, three segmental duplications, four tandem duplications and 11 whole-genome duplications occurred among the CsPrxs, contributing to the family size expansion. From the Ka/Ks ratios, 15 of 18 duplicated CsPrxs pairs have experienced purifying selection process. A total of 15 conserved motifs were detected in CsPrxs, four of which were detected in all complete CsPrxs. A total of 12 expressed genes were identified from the EST database. The expression trends of 12 CsPrxs were differently expressed at different stages of infection by Xcc, five of which were potential candidate genes involved in Xcc resistance. These genes could be induced by salicylic acid and methyl jasmonate, and were extracellular proteins. These results further support our understanding of CIII prxs in citrus, particularly in citrus bacterial canker studiesen_US
dc.identifier.affiliationsCitrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400712, People’s Republic of Chinaen_US
dc.identifier.citationLi Qiang, Dou Wanfu, Qi Jingjing, Qin Xiujuan, Chen Shanchun, He Yongrui. Genomewide analysis of the CIII peroxidase family in sweet orange (Citrus sinensis) and expression profiles induced by Xanthomonas citri subsp. citri and hormones. Journal of Genetics. 2020 Jan; 99: 1-13en_US
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215554
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume99en_US
dc.source.urihttps://doi.org/10.1007/s12041-019-1163-5en_US
dc.subjectsweet orangeen_US
dc.subjectclass III peroxidaseen_US
dc.subjectcitrus bacterial cankeren_US
dc.subjectgene duplicationsen_US
dc.subjectXanthomonas citri subsp. citri (Xcc).en_US
dc.titleGenomewide analysis of the CIII peroxidase family in sweet orange (Citrus sinensis) and expression profiles induced by Xanthomonas citri subsp. citri and hormonesen_US
dc.typeJournal Articleen_US
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