Cockroach allergen detection and cockroach allergens of allergic Thai patients.

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dc.contributor.authorSookrung, Nitaten_US
dc.contributor.authorDiraphat, Pornphanen_US
dc.contributor.authorChaicumpa, Wanpenen_US
dc.contributor.authorTongtawe, Pongsrien_US
dc.contributor.authorSakolvaree, Yuwapornen_US
dc.contributor.authorTapchaisri, Pramuanen_US
dc.contributor.authorMahakittikun, Vannaen_US
dc.contributor.authorTungtrongchitr, Anchaleeen_US
dc.contributor.authorVichyanond, Pakiten_US
dc.contributor.authorBunnag, Chaweewanen_US
dc.date.accessioned2009-05-27T17:25:37Z
dc.date.available2009-05-27T17:25:37Z
dc.date.issued2003-03-23en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractHybridomas secreting monoclonal antibodies (MAb) specific to American cockroach (Periplaneta americana) were produced through a fusion of immune splenocytes of a BALB/c mouse immunized with crude cockroach (CR) extract and mouse myeloma cells. Two hybridomas namely 38G6 and 3C2 were established. These specific hybridomas secreted IgG1 monoclonal immunoglobulins with antigenic specificities to CR protein components of over 207 to 72 kDa and 45 to 40 kDa, respectively. The monoclonal antibodies were applied to select their specific epitopes out of the crude CR extract using affinity chromatography. A Prausnitz-Kustner test revealed that these epitopes were allergens which caused wheals and flares of the skin of a guinea-pig previously sensitized with a pool of serum samples from CR allergic patients. The monoclonal antibodies were also used in a capture ELISA to detect specific IgE in serum samples of allergic Thai patients. It was found that 72% and 76% of the patients had IgE antibodies to the epitopes of MAb 38G6 and MAb 3C2, respectively, indicating that the two epitopes are major CR allergens among the CR allergic Thai patients. An antibody-sandwich ELISA was developed for quantitative detection of CR allergens using the two monoclonal antibodies as a capture reagent and rabbit polyclonal antibodies to crude CR extract as a detection reagent. The assay could detect allergenic epitopes contained in as little as 122 pg of crude cockroach extract, and has high potential for direct measurement of the marker allergens in extracts of environmental samples.en_US
dc.description.affiliationFaculty of Allied Health Sciences, Thammasat University, Rangsit Center, Pathum-thani, Thailand.en_US
dc.identifier.citationSookrung N, Diraphat P, Chaicumpa W, Tongtawe P, Sakolvaree Y, Tapchaisri P, Mahakittikun V, Tungtrongchitr A, Vichyanond P, Bunnag C. Cockroach allergen detection and cockroach allergens of allergic Thai patients. Asian Pacific Journal of Allergy and Immunology. 2003 Mar; 21(1): 1-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/37167
dc.language.isoengen_US
dc.subject.meshAllergens --immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal --immunologyen_US
dc.subject.meshChilden_US
dc.subject.meshCockroaches --immunologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gel --methodsen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshHybridomas --immunologyen_US
dc.subject.meshHypersensitivity --immunologyen_US
dc.subject.meshIntradermal Tests --methodsen_US
dc.subject.meshMiceen_US
dc.subject.meshRabbitsen_US
dc.subject.meshThailanden_US
dc.titleCockroach allergen detection and cockroach allergens of allergic Thai patients.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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