Seroreactivity of 31 kDa and 41 kDa mycobacterial secretory proteins isolated from culture filtrate in extra pulmonary tuberculosis.

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dc.contributor.authorBanerjee, Swatien_US
dc.contributor.authorGupta, Sonikaen_US
dc.contributor.authorKumar, Satishen_US
dc.contributor.authorShrikhande, A Ven_US
dc.contributor.authorReddy, M V Ren_US
dc.contributor.authorHarinath, B Cen_US
dc.date.accessioned2003-04-17en_US
dc.date.accessioned2009-05-29T13:17:03Z
dc.date.available2003-04-17en_US
dc.date.available2009-05-29T13:17:03Z
dc.date.issued2003-04-17en_US
dc.description.abstractDespite rapid advances in molecular genetics for detection of mycobacteria, it is clear that interest in serodiagnosis remains high, especially for those situations in which a specimen may not contain the infecting agent in particular in extrapulmonary tuberculosis. Immune response to excretory-secretory (ES) proteins of Mycobacterium tuberculosis (M.tb) has been of diagnostic interest in tuberculosis. In earlier study from our laboratory, a secretory protein M.tb ES-31 has been shown to have diagnostic potential in pulmonary tuberculosis. Further, another M.tb H37Ra ES protein (ES-41) was isolated and purified by trichloroacetic acid solubilization followed by Fast Performance Liquid Chromatography (FPLC). These two protein fractions viz ES-31 and ES-41 secreted by M.tb H37 Ra bacilli were employed in stick indirect penicillinase ELISA to study seroreactivity in extra pulmonary tuberculosis namely tuberculous lymphadenopathy, tuberculous meningitis, abdominal tuberculosis and bone & joint tuberculosis. While using ES-31 antigen 88% (22/25) of tuberculous lymphadenopathy and 90% (9/10) of tuberculous meningitis cases showed positive reaction for tuberculous IgG antibody, ES-41 showed 80% positivity in both groups. In abdominal and bone & joint tuberculosis cases, ES-41 antigen showed better sensitivity of 81.5% (22/27) and 84.6% (22/26) respectively in IgG antibody detection compared to 70% (19/27) and 69.2% (18/26) shown by ES-31. This study is of interest that different antigen protein fractions of M.tb exhibit differential seroreactivity, as ES-31 protein showed good potential in detecting tuberculous IgG antibodies in tuberculous lymphadenopathy (TBLN) & tuberculous meningitis (TBM), while ES-41 in abdominal and bone & joint tuberculosis cases.en_US
dc.description.affiliationJamnalal Bajaj Tropical Disease Research Centre, Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha.en_US
dc.identifier.citationBanerjee S, Gupta S, Kumar S, Shrikhande AV, Reddy MV, Harinath BC. Seroreactivity of 31 kDa and 41 kDa mycobacterial secretory proteins isolated from culture filtrate in extra pulmonary tuberculosis. Indian Journal of Pathology & Microbiology. 2003 Apr; 46(2): 261-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/75486
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshAntigens, Bacterial --chemistryen_US
dc.subject.meshBacterial Proteins --chemistryen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --blooden_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshMycobacterium tuberculosis --immunologyen_US
dc.subject.meshSerologic Testsen_US
dc.subject.meshTuberculosis --diagnosisen_US
dc.titleSeroreactivity of 31 kDa and 41 kDa mycobacterial secretory proteins isolated from culture filtrate in extra pulmonary tuberculosis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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