Detection of M. leprae by reverse transcription- PCR in biopsy specimens from leprosy cases: a preliminary study.

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dc.contributor.authorHirawati,en_US
dc.contributor.authorKatoch, Ken_US
dc.contributor.authorChauhan, D Sen_US
dc.contributor.authorSingh, H Ben_US
dc.contributor.authorSharma, V Den_US
dc.contributor.authorSingh, Men_US
dc.contributor.authorKashyap, Men_US
dc.contributor.authorKatoch, V Men_US
dc.date.accessioned2006-03-22en_US
dc.date.accessioned2009-06-01T18:04:57Z
dc.date.available2006-03-22en_US
dc.date.available2009-06-01T18:04:57Z
dc.date.issued2006-03-22en_US
dc.description.abstractA reverse transcription (RT)-PCR assay targeting 16S rRNA of Mycobacterium leprae has been used to detect M.leprae specific nucleic acids. This study has been initiated to gain experience about detection of RNA from seven biopsy specimens by RT-PCR assay using species- specific primers described earlier. These biopsy specimens were from clinically confirmed and untreated leprosy cases belonging to BB and BL types. The earlier reported method was established in our laboratory. 171 bp fragment by RT-PCR was amplified from 4/7 cases. The positives results by RT-PCR were from the biopsies from fresh or short term treated cases whereas negative results were from specimens from long term treated cases showing clinical features of relapse. DNA targeting PCR (36 KDa) showed positivity in both groups. These results suggest that RT-PCR positivity possibly reflect the presence of viable organisms. Thus as earlier predicted RT-PCR assay may be useful for viability determinations for assessing the response to chemotherapy as well as presence of persisters in relapse cases.en_US
dc.description.affiliationNational JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Tajganj, Agra (Uttar Pradesh)-282001-India.en_US
dc.identifier.citationHirawati , Katoch K, Chauhan DS, Singh HB, Sharma VD, Singh M, Kashyap M, Katoch VM. Detection of M. leprae by reverse transcription- PCR in biopsy specimens from leprosy cases: a preliminary study. Journal of Communicable Diseases. 2006 Mar; 38(3): 280-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111965
dc.language.isoengen_US
dc.subject.meshBiopsyen_US
dc.subject.meshHumansen_US
dc.subject.meshLeprosy --diagnosisen_US
dc.subject.meshMycobacterium leprae --geneticsen_US
dc.subject.meshRNA, Ribosomal, 16S --classificationen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSkin --microbiologyen_US
dc.titleDetection of M. leprae by reverse transcription- PCR in biopsy specimens from leprosy cases: a preliminary study.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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