Significance of the hepatitis C virus (HCV) core antigen as an alternative plasma marker of active HCV infection.

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dc.contributor.authorDaniel, H D Jen_US
dc.contributor.authorVivekanandan, Pen_US
dc.contributor.authorRaghuraman, Sen_US
dc.contributor.authorSridharan, Gen_US
dc.contributor.authorChandy, G Men_US
dc.contributor.authorAbraham, Pen_US
dc.date.accessioned2007-01-23en_US
dc.date.accessioned2009-05-28T09:20:40Z
dc.date.available2007-01-23en_US
dc.date.available2009-05-28T09:20:40Z
dc.date.issued2007-01-23en_US
dc.description.abstractPURPOSE: To evaluate the role of core antigen (Ortho trak-C assay) as a marker of active HCV infection in comparison to HCV RNA as detected by reverse transcription polymerase chain reaction (RT-PCR). METHODS: This evaluation was carried out during January 2000 to December 2003 in HCV infected individuals who were treatment naomicronve or were on anti-viral therapy. Additionally, sequential plasma samples from patients on clinical follow-up were included in this study. A total of 167 samples from 61 patients were tested by trak-C and RT-PCR. HCV RNA detection was achieved by a RT-PCR. Trak-C assay results were also compared in a limited proportion of these samples with known HCV viral load and genotype. RESULTS: Of 167 samples tested, 56.9% were RNA positive and 43.1% were RNA negative while 50.3% were trak-C positive and 49.7% were trak-C negative, yielding a sensitivity of 85.3% and a specificity of 95.8% for the trak-C assay (Kappa co-efficient = 0.8). The concentration of HCVcAg and HCV RNA showed significant correlation (n=38, r=0.334, P =0.04). The trak-C assay detected the most prevalent HCV genotypes in India without significant difference (P =0.335). The difference between mean absorbance values of HCV RNA positive samples compared to HCV RNA negative samples in the trak-C assay was highly significant (P < 0.000). Qualitative results of trak-C assay and RT-PCR were comparable in 93% of follow-up samples. CONCLUSIONS: Trak-C assay can be recommended for confirmation of HCV infection and follow-up in laboratories with resource-poor facilities.en_US
dc.description.affiliationDepartment of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India.en_US
dc.identifier.citationDaniel HD, Vivekanandan P, Raghuraman S, Sridharan G, Chandy GM, Abraham P. Significance of the hepatitis C virus (HCV) core antigen as an alternative plasma marker of active HCV infection. Indian Journal of Medical Microbiology. 2007 Jan; 25(1): 37-42en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/53786
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshHepacivirus --geneticsen_US
dc.subject.meshHepatitis C --blooden_US
dc.subject.meshHepatitis C Antigens --blooden_US
dc.subject.meshIndiaen_US
dc.subject.meshRNA, Viral --geneticsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleSignificance of the hepatitis C virus (HCV) core antigen as an alternative plasma marker of active HCV infection.en_US
dc.typeJournal Articleen_US
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