Necessity of nuclear and mitochondrial genome analysis prior to assisted reproductive techniques/intracytoplasmic sperm injection.

Simple item page
dc.contributor.authorKumar, Rakeshen_US
dc.contributor.authorBhat, Audeshen_US
dc.contributor.authorBamezai, R N Ken_US
dc.contributor.authorShamsi, M Ben_US
dc.contributor.authorKumar, Ren_US
dc.contributor.authorGupta, N Pen_US
dc.contributor.authorAmmini, A Cen_US
dc.contributor.authorAron, Men_US
dc.contributor.authorSharma, R Ken_US
dc.contributor.authorDada, Rimaen_US
dc.date.accessioned2007-12-07en_US
dc.date.accessioned2009-05-27T09:43:22Z
dc.date.available2007-12-07en_US
dc.date.available2009-05-27T09:43:22Z
dc.date.issued2007-12-07en_US
dc.description.abstractAssisted reproductive technique (ART) has revolutionized the management of severe male factor infertility and in some countries 5% babies are conceived through ART/intra cytoplasmic sperm injection (ICSI). However, the carry-home live birth rate after several ART cycles is low (18-25%) and this is financially, physically and emotionally crippling for the couples. Genetic factors could lead to pre or post-implantation failure and thus explain for low ART success rate. Thus, this study was planned to understand, if infertile men harbour genetic abnormalities which may be iatrogenically transmitted by ART and adversely affect growth potential of embryo. Ninety infertile men underwent semen, cytogenetic, Yq microdeletion and mitochondrial mutation analysis. Of these, 14.4% cases harboured cytogenetic abnormality, and 8.89% Yq microdeletions. A high frequency of mitochondrial mutations was found in 23 men with asthenospermia. It is important to understand that through ART genetic abnormalities are transmitted to offspring, resulting in impaired growth and development potential of embryo and poor take-home live birth rate. Thus, genetic analysis is strongly recommend in all men with idiopathic infertility who opt for ART to counsel couples and provide them with most adapted therapeutics.en_US
dc.description.affiliationLaboratory of Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), N. Delhi 110029, India.en_US
dc.identifier.citationKumar R, Bhat A, Bamezai RN, Shamsi MB, Kumar R, Gupta NP, Ammini AC, Aron M, Sharma RK, Dada R. Necessity of nuclear and mitochondrial genome analysis prior to assisted reproductive techniques/intracytoplasmic sperm injection. Indian Journal of Biochemistry & Biophysics. 2007 Dec; 44(6): 437-42en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28365
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAdulten_US
dc.subject.meshCell Nucleus --geneticsen_US
dc.subject.meshChromosome Deletionen_US
dc.subject.meshChromosomes, Human, Y --geneticsen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGenomeen_US
dc.subject.meshHumansen_US
dc.subject.meshInfertility, Male --diagnosisen_US
dc.subject.meshMaleen_US
dc.subject.meshMitochondria --geneticsen_US
dc.subject.meshMutationen_US
dc.subject.meshReproductive Techniques, Assisteden_US
dc.subject.meshSemen --chemistryen_US
dc.subject.meshSperm Injections, Intracytoplasmicen_US
dc.titleNecessity of nuclear and mitochondrial genome analysis prior to assisted reproductive techniques/intracytoplasmic sperm injection.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics