FLT3 and NPM-1 mutations in a cohort of acute promyelocytic leukemia patients from India.
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Date
2014-04
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Abstract
Background: Acute promyelocytic leukemia (APL)
with t (15;17) is a distinct category of acute myeloid
leukemia (AML) and is reported to show better response
to anthracyclin based chemotherapy. A favorable overall
prognosis over other subtypes of AML has been reported
for APL patients but still about 15% patients relapse.
Methods: This study evaluated the presence of Famus
like tyrosine kinase‑3 (FLT3) and nucleophosmin‑1 (NPM1)
gene mutations in a cohort of 40 APL patients. Bone
marrow/peripheral blood samples from patients at the
time of diagnosis and follow‑up were processed for
immunophenotyping, cytogenetic markers and isolation of
DNA and RNA. Samples were screened for the presence
of mutations in FLT3 and NPM1 genes using polymerase
chain reaction followed by sequencing.
Results: Frequency of FLT3/internal tandem duplication
and FLT3/tyrosine kinase domain was found to be 25% and
7% respectively. We observed a high frequency of NPM1
mutation (45%) in the present population of APL patients.
Description
Keywords
Acute promyelocytic leukemia, famus like tyrosine kinase‑3, nucleophosmin‑1
Citation
Swaminathan Suchitra, Garg Swati, Madkaikar Manisha, Gupta Maya, Jijina Farah, Ghosh Kanjaksha. FLT3 and NPM-1 mutations in a cohort of acute promyelocytic leukemia patients from India. Indian Journal of Human Genetics. 2014 Apr-Jun ; 20 (2): 160-165.