In vitro and in vivo inhibition of telomerase activity from Chinese hamster V79 cells.

Simple item page
dc.contributor.authorPandit, Ben_US
dc.contributor.authorBhattacharyya, N Pen_US
dc.date.accessioned2001-02-21en_US
dc.date.accessioned2009-05-27T09:53:24Z
dc.date.available2001-02-21en_US
dc.date.available2009-05-27T09:53:24Z
dc.date.issued2001-02-21en_US
dc.description.abstractWhile studying the inhibition of telomerase activity in Chinese hamster V79 cells using polymerase chain reaction (PCR) based telomeric repeat amplification protocol (TRAP) assay, we had earlier observed that 7-deaza deoxy guanosine triphosphate (7-deaza dGTP) and oligonucleotide (TTAGGG)4 inhibited telomerase activity in vitro. In the present study, we report inhibition of telomerase activity by modified base 7-deaza deoxy adenosine triphosphate (7-deaza dATP) and phosphorothioate TTAGGG (PS-TTAGGG). Both the compounds inhibited telomerase activity in a concentration dependent manner; 8.5 microM of 7-deaza dATP and 0.1 microM of PS-TTAGGG being the concentration for 50% of the maximum inhibition. This observation supports our earlier hypothesis that incorporation of a modified nucleotide into telomere possibly interferes with the recognition of the telomerase and TTAGGG interferes with the RNA component of telomerase. We have further shown that treatment of cells with nicotinamide (NA) and benzamide (BA), well known inhibitors of poly (ADP-ribose) polymerase, reduced telomerase activity. We speculate that modification of the telomeric binding proteins or other components by poly (ADP-ribosyl)ation may be involved in such inhibition.en_US
dc.description.affiliationCrystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Calcutta 700 064, India.en_US
dc.identifier.citationPandit B, Bhattacharyya NP. In vitro and in vivo inhibition of telomerase activity from Chinese hamster V79 cells. Indian Journal of Biochemistry & Biophysics. 2001 Feb-Apr; 38(1-2): 42-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28864
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBenzamides --pharmacologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshCricetulusen_US
dc.subject.meshDensitometryen_US
dc.subject.meshDeoxyguanine Nucleotides --pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshNiacinamide --pharmacologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshTelomerase --antagonists & inhibitorsen_US
dc.subject.meshTelomere --metabolismen_US
dc.titleIn vitro and in vivo inhibition of telomerase activity from Chinese hamster V79 cells.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics