Purification and properties of proteases produced by Alternaria alternata (Fr.) Keissl, regulation of production by fructose.
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Date
1985-09
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Abstract
A strain οf Alternaria alternata (Fr.) Keissl, when grown on wheat bran Czapek Dox
medium was found to secrete one neutral and two alkaline proteases. The purified enzymes
were found to be endo peptidases, the alkaline proteases being serine proteases and neutral
proteases being cysteine proteases. Fructose when added to the culture medium was found to
give rise to a new neutral protease at the expense of the neutral protease produced in the
absence of fructose and was also found to enhance the production of alkaline proteases. It also
appears that fructose modifies the alkaline proteases with respect to some characteristics such
as Vmax, Ea etc. Sodium dodecyl sulphate Polyacrylamide gel electrophoresis indicated a
significantly altered protein profile in fructose supplemented .medium.
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Keywords
Alternaria, proteases, fructose
Citation
Patil M, Shastri N V. Purification and properties of proteases produced by Alternaria alternata (Fr.) Keissl, regulation of production by fructose. Journal of Biosciences. 1985 Sept; 9(1&2): 1-11.