Antimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp.

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dc.contributor.authorHandayani, Dianen_US
dc.contributor.authorAnanda, Nitaen_US
dc.contributor.authorMuh., Ade Artasastaen_US
dc.contributor.authorRuslan, Rustinien_US
dc.contributor.authorFadriyanti, Okmesen_US
dc.contributor.authorTrina, Ekawati Talleien_US
dc.date.accessioned2020-10-16T08:55:57Z
dc.date.available2020-10-16T08:55:57Z
dc.date.issued2019-04
dc.description.abstractThis study aims to evaluate the antimicrobial activities of endophytic fungi from marine brown algae Padina sp.collected from Nirwana Beach, Padang, West Sumatera, Indonesia. The isolation of endophytic fungi was conductedusing dilution method with Sabouraud Dextrose Agar (SDA) + Chloramphenicol as a growth medium. Nine fungistrains have been isolated from this alga. Purely isolated fungi were cultivated using rice as a medium at roomtemperature for 3–4 weeks. The secondary metabolite produced by fungi was extracted using ethyl acetate (EtOAc)as a solvent. The antimicrobial activity of EtOAc extracts was tested against Staphylococcus aureus, Escherichiacoli, and Candida albicans by using the agar diffusion method. In this research, nine endophytic fungi were isolatedfrom the brown marine algae Padina sp. The results of antimicrobial activity screening showed that one fungal isolate(Nita3) was selected as the most active against S. aureus, E. coli, and C. albicans with a diameter of inhibition zone of20.98 ± 1.56 mm, 17.98 ± 6.58 mm, and 13.60 ± 0 mm, respectively. This selected fungus was identified molecularlyas Trichoderma harzianum. We conclude that T. harzianum can be a source of antimicrobial compounds. However,continuous research is needed to prove its bioactive actionen_US
dc.identifier.affiliationsSumatran Biota Laboratory, Faculty of Pharmacy, University of Andalas, Kampus Limau Manis, Padang, Indonesiaen_US
dc.identifier.affiliationsSumatran Biota Laboratory, Faculty of Pharmacy, University of Andalas, Kampus Limau Manis, Padang, Indonesia.en_US
dc.identifier.affiliationsSumatran Biota Laboratory, Faculty of Pharmacy, University of Andalas, Kampus Limau Manis, Padang, Indonesia.en_US
dc.identifier.affiliationsFaculty of Dentistry, University of Baiturahmah, Padang, Indonesiaen_US
dc.identifier.affiliationsDepartment of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, Indonesiaen_US
dc.identifier.citationHandayani Dian, Ananda Nita, Muh. Ade Artasasta, Ruslan Rustini, Fadriyanti Okmes, Trina Ekawati Tallei. Antimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp.. Journal of Applied Pharmaceutical Science. 2019 Apr; 2019 Apr: 009-013en_US
dc.identifier.issn2231-3354
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/210558
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber4en_US
dc.relation.volume9en_US
dc.source.urihttps://dx.doi.org//10.7324/JAPS.2019.90302en_US
dc.subjectAntimicrobial activityen_US
dc.subjectbrown algaeen_US
dc.subjectmarine algaederived fungien_US
dc.subjectmolecular characterizationen_US
dc.subjectTrichoderma harzianumen_US
dc.subjectPadina spen_US
dc.titleAntimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp.en_US
dc.typeJournal Articleen_US
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