Seminal LDH-C4 isoenzyme and sperm mitochondrial activity: a study in male partners of infertile couples.

Simple item page
dc.contributor.authorSawane, M Ven_US
dc.contributor.authorKaore, S Ben_US
dc.contributor.authorGaikwad, R Den_US
dc.contributor.authorPatil, P Men_US
dc.contributor.authorPatankar, S Sen_US
dc.contributor.authorDeshkar, A Men_US
dc.date.accessioned2002-11-27en_US
dc.date.accessioned2009-05-29T06:05:05Z
dc.date.available2002-11-27en_US
dc.date.available2009-05-29T06:05:05Z
dc.date.issued2002-11-27en_US
dc.description.abstractLactate dehydrogenase isoenzyme patterns were obtained in the seman of 93 male partners of infertile couples and 28 proven fertile subjects as a control group. Sperm mitochondrial activity index (SMAI) alongwith the conventional seminal parameters was studied for all the subjects excepting the azoospermic and vasectomised males. Only LDH-C4, a germ cell specific isoenzyme activity varied with the variation in sperm density. LDH-C4 activity varied significantly (p<0.001) within and between different groups. Lactate dehydrogenase-C4 activity was absent in 17 azoospermic samples, confirming its germinal epithelial origin, as well as in 8 samples of vasectomised males. In one azoospermic sample, there were many immature germ cells along with surprisingly high LDH-C4 activity suggesting more activity of germinal epithelium associated with high LDH-C4 activity. LDH-C4 activity was reduced significantly in oligozoospermic samples in proportion to sperm density, thus confirming strong correlation (p<0.001) between LDH-C4 and sperm density. There was statistically significant correlation between LDH-C4 and percentage sperm motility as well as between LDH-C4 and Sperm Mitochondrial Activity Index (SMAI) (probability varying from p<0.05 to p<0.01 in different groups), but no such correlation was found between LDH-C4 and sperm morphology. The data confirms LDH-C4 as a germinal epithelial marker. Its relationship with percentage sperm motility is suggestive of definite role of LDH-C4 in evaluation of the spermatozoal quality, similarly its relationship with Sperm Mitochondrial Activity Index (SMAI score) suggest the role of LDH-C4 in metabolism of the spermatocytes and sperms, though further studies are required for clear and detailed understanding of its metabolic role in semen.en_US
dc.description.affiliationDepartment of Physiology, Government Medical College, Nagpur-440003.en_US
dc.identifier.citationSawane MV, Kaore SB, Gaikwad RD, Patil PM, Patankar SS, Deshkar AM. Seminal LDH-C4 isoenzyme and sperm mitochondrial activity: a study in male partners of infertile couples. Indian Journal of Medical Sciences. 2002 Nov; 56(11): 560-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/68401
dc.language.isoengen_US
dc.source.urihttps://www.indianjmedsci.orgen_US
dc.subject.meshAnalysis of Varianceen_US
dc.subject.meshHumansen_US
dc.subject.meshInfertility, Male --enzymologyen_US
dc.subject.meshIsoenzymes --metabolismen_US
dc.subject.meshL-Lactate Dehydrogenase --metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMitochondria --enzymologyen_US
dc.subject.meshOligospermia --enzymologyen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshSperm Counten_US
dc.subject.meshSperm Motility --physiologyen_US
dc.subject.meshSpermatozoa --enzymologyen_US
dc.titleSeminal LDH-C4 isoenzyme and sperm mitochondrial activity: a study in male partners of infertile couples.en_US
dc.typeClinical Trialen_US
dc.typeControlled Clinical Trialen_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Medical Sciences