Deconvolution of the fluorescence spectra into its component Gaussians: a method to analyze the binding of coumarin to bovine serum albumin.

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dc.contributor.authorBharathi, Sen_US
dc.contributor.authorMishra, A Ken_US
dc.date.accessioned1999-06-29en_US
dc.date.accessioned2009-05-27T09:03:23Z
dc.date.available1999-06-29en_US
dc.date.available2009-05-27T09:03:23Z
dc.date.issued1999-06-29en_US
dc.description.abstract7-N,N-Diethylamino-4-methylcoumarin, (cou-1), a readily available laser dye binding to bovine serum albumin (BSA), at room temperature has been studied by steady state fluorescence spectroscopy. Existing methods of analysis of the binding to obtain the binding parameters are based on the change in fluorescence intensity at a particular wavelength. These methods are not convenient when there is a gradual shift in the emission maxima for increasing protein concentration. In this paper we present a method to obtain the binding constants of cou-1 to BSA using a Windows '95 based package to deconvolute the asymmetrical spectrum (fluorescence intensity versus wave number curve) into two Gaussians, each corresponding to the binding of the fluorophore to a particular site. This method is convenient to analyze the binding constant data and obtain the binding parameters of each binding site, and can also provide information about the microenvironment of each site, relating micropolarity and microviscosity.en_US
dc.description.affiliationDepartment of Chemistry, Indian Institute of Technology, Chennai.en_US
dc.identifier.citationBharathi S, Mishra AK. Deconvolution of the fluorescence spectra into its component Gaussians: a method to analyze the binding of coumarin to bovine serum albumin. Indian Journal of Biochemistry & Biophysics. 1999 Jun; 36(3): 188-94en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26304
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshCoumarins --metabolismen_US
dc.subject.meshFluorescence Polarization --methodsen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshSerum Albumin, Bovine --metabolismen_US
dc.titleDeconvolution of the fluorescence spectra into its component Gaussians: a method to analyze the binding of coumarin to bovine serum albumin.en_US
dc.typeJournal Articleen_US
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