Sperm motility after passage through the hen's egg yolk medium.

dc.contributor.authorChinsomboon, Sen_US
dc.contributor.authorNattharit, Pen_US
dc.contributor.authorWongkularb, Aen_US
dc.contributor.authorSudsam-Ang, Aen_US
dc.contributor.authorOsathanondh, Ven_US
dc.date.accessioned2009-05-27T19:28:09Z
dc.date.available2009-05-27T19:28:09Z
dc.date.issued1990-02-01en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractThis study aimed to observe the percentage of motility of human spermatozoa after passage through 20 per cent hen's egg yolk medium. Twenty-one samples of freshly ejaculated human semen having a sperm density over 20 million per ml and a percentage of motile sperm over 50 per cent were used in the study. The percentage of sperm motility was estimated under the microscope by conventional method. The sperm motility was evaluated within 30 minutes after ejaculation and every one hour thereafter for 8 hours. After the first observation, the semen was divided into two parts, one part kept as a control and the other used for the experiment. The latter was washed in Tyrode's solution and overlaid on 20 per cent egg yolk column and allowed to migrate toward the bottom of the column for two and a half hours. The spermatozoa at the bottom of the column were brought out and washed in Tyrode's solution. The percentage of the sperm motility was observed every hour for 5 hours. After passing through the 20 per cent egg yolk medium, percentage of sperm motility was observed to increase from 66.2 to 87.2 while that of the control declined to 49.3. Five hours after passing through the egg yolk medium the percentage of motile sperm was still high, an average of 80 as compared to 17.4 for the control. Results of this study indicate that hen's egg yolk has a sperm motility stimulating property. Whether hen's egg yolk can be used as a sperm motility stimulant in the treatment of such conditions as asthenospermia and oligospermia is subjected for further study.en_US
dc.description.affiliationDepartment of Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationChinsomboon S, Nattharit P, Wongkularb A, Sudsam-Ang A, Osathanondh V. Sperm motility after passage through the hen's egg yolk medium. Journal of the Medical Association of Thailand. 1990 Feb; 73 Suppl 1(): 100-3en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/41186
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChickensen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshEgg Yolken_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshSperm Motility --physiologyen_US
dc.titleSperm motility after passage through the hen's egg yolk medium.en_US
dc.typeJournal Articleen_US
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