Isolation and characterization of alpha-galactosidase from coconut endosperm

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dc.contributor.authorMATHEW, CPDWen_US
dc.date.accessioned2011-02-14T15:29:50Z
dc.date.available2011-02-14T15:29:50Z
dc.date.created1985en_US
dc.date.issued1985en_US
dc.descriptionDissertation: PhD, University of Colombo: UC(MED), 1985.en_US
dc.description.abstractAlpha- galactosidase extracted from coconut endosperm was purified to homogenity as determined by polyacrylamide gel electrophoresis by affinity chromatography using adsorbants. Large scale purification was done by hydrophobic chromatography. Purified alpha galactosidase was reduced alkylated, and fragment. The peptides obtained were used for amino acid analysis. Inhibition of alpha galactosidase was seen by N-Bromosuccinamide, oxidation of two tryptophan residues, Modification of thyrosine residue and carbodiamide. But in the presence of the competitive inhibitor D- galactose the enzyme was prolected from inhibition.en_US
dc.identifier.citationMATHEW, CPDW, Isolation and characterization of alpha-galactosidase from coconut endosperm, University of Colombo UC(MED), 1985: 288p.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/129602
dc.language.isoen_USen_US
dc.publisherUniversity of Colombo: UC(MED).en_US
dc.rightsUniversity of Colombo, UC(MED): Sri Lanka HELLIS Networken_US
dc.source.urihttps://hellis.srilanka.healthrepository.orgen_US
dc.subjectEnzymesen_US
dc.subject.meshGalactosidasesen_US
dc.subject.meshalpha-Galactosidaseen_US
dc.subject.meshalpha-Galactosidase-isolation \& purificationen_US
dc.subject.meshChromotographyen_US
dc.titleIsolation and characterization of alpha-galactosidase from coconut endospermen_US
dc.typeThesisen_US
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