Novel real-time PCr for detection of Schistosoma japonicum in stool.

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dc.contributor.authorLier, Ten_US
dc.contributor.authorSimonsen, G Sen_US
dc.contributor.authorHaaheim, Hen_US
dc.contributor.authorHjelmevoll, S Oen_US
dc.contributor.authorVennervald, B Jen_US
dc.contributor.authorJohansen, M Ven_US
dc.date.accessioned2009-05-27T15:00:16Z
dc.date.available2009-05-27T15:00:16Z
dc.date.issued2006-03-28en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractChemotherapy has been used on a large scale in countries where the blood fluke Schistosoma japonicum is endemic. This has led to a lower intensity of infections and consequently lower diagnostic values of commonly used diagnostic tests like serology and Kato-Katz stool smear. We designed a novel real-time PCR method for detection of S. japonicum in stool samples. Further, we evaluated different versions of an inexpensive, non-commercial extraction method, ROSE, as well as the commercial QIAamp DNA Stool Mini Kit. PCR primer sequences were designed targeting the mitochondrial NADH dehydrogenase I gene. Bovine serum albumin was added to the DNA extracts and SYBR Green was used for detection. The PCR method was evaluated with non-infected stool samples spiked with S. japonicum eggs. It demonstrated high sensitivity, even in samples containing a single egg. The two extraction methods were equally effective. The PCR was specific for S. japonicum when tested against other Schistosoma species, Trichuris trichiura, hookworm and Taenia sp. We conclude that this novel real-time PCR, in combination with either ROSE or QIAamp DNA Stool Mini Kit extraction, is a sensitive and specific tool for diagnosing S. japonicum in human stool samples.en_US
dc.description.affiliationDepartment of Microbiology, University and University Hospital of North Norway, Tromsø, Norway. torelier@online.noen_US
dc.identifier.citationLier T, Simonsen GS, Haaheim H, Hjelmevoll SO, Vennervald BJ, Johansen MV. Novel real-time PCr for detection of Schistosoma japonicum in stool. The Southeast Asian Journal of Tropical Medicine and Public Health. 2006 Mar; 37(2): 257-64en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/31662
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2006_37_2/03-3713.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDNA, Helminth --chemistryen_US
dc.subject.meshDiagnosis, Differentialen_US
dc.subject.meshFeces --parasitologyen_US
dc.subject.meshHumansen_US
dc.subject.meshParasite Egg Counten_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshRose Bengal --diagnostic useen_US
dc.subject.meshSchistosoma japonicum --isolation & purificationen_US
dc.subject.meshSchistosomiasis japonica --diagnosisen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSpecies Specificityen_US
dc.titleNovel real-time PCr for detection of Schistosoma japonicum in stool.en_US
dc.typeJournal Articleen_US
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