Activity and stability of Bacillus cereus penicillinase entrapped in aerosol OT reverse micelles.

Simple item page
dc.contributor.authorChakravarty, Ken_US
dc.contributor.authorVarshney, Men_US
dc.contributor.authorMaitra, Aen_US
dc.date.accessioned1995-04-01en_US
dc.date.accessioned2009-05-27T09:13:11Z
dc.date.available1995-04-01en_US
dc.date.available2009-05-27T09:13:11Z
dc.date.issued1995-04-01en_US
dc.description.abstractThe kinetics of enzyme catalyzed hydrolysis of penicillin G and stability of the enzyme alpha-penicillinase, entrapped in aerosol OT reverse micellar droplets have been investigated spectrophotometrically. Various physical parameters, such as, water pool size (related to Wo), pH and temperature, were optimized for maximum activity of penicillinase in water/aerosol OT/isooctane reverse micelles. The enzyme showed maximum activity of Wo - 14 and pH, 7.0. At any temperature the enzyme was to be more active in reverse micelles than in aqueous solution. At optimum conditions of Wo, pH and temperature the enzyme was 100% more active in reverse micelles than its maximum activity in aqueous solution. In both the systems, the activity starts falling at and above 25 degrees C. CD Spectral studies showed that the enzyme in reverse micelles possesses more helical structure than it has in aqueous solution and at the optimum conditions in which it showed maximum activity, the alpha-helicity was also maximum. The enzyme was very stable in reverse micelles at and above room temperature compared to the same in aqueous solution.en_US
dc.description.affiliationDepartment of Chemistry, University of Delhi, India.en_US
dc.identifier.citationChakravarty K, Varshney M, Maitra A. Activity and stability of Bacillus cereus penicillinase entrapped in aerosol OT reverse micelles. Indian Journal of Biochemistry & Biophysics. 1995 Apr; 32(2): 100-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26833
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshBacillus cereus --enzymologyen_US
dc.subject.meshDioctyl Sulfosuccinic Aciden_US
dc.subject.meshEnzyme Stabilityen_US
dc.subject.meshKineticsen_US
dc.subject.meshMicellesen_US
dc.subject.meshPenicillinase --metabolismen_US
dc.titleActivity and stability of Bacillus cereus penicillinase entrapped in aerosol OT reverse micelles.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics