Ethanol-induced changes in lipid peroxidation of enterocytes across the crypt–villus axis in rats.
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Date
2010-01
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Abstract
Background Reactive oxygen species (ROS) have been implicated in the turnover of epithelial cells in the rat intestine.
The metabolism of ethanol generates ROS, which are implicated
in cellular injury, but the levels of lipid peroxidation in
intestine in chronic alcoholism are unknown.
Aim To investigate the effects of ethanol ingestion on lipid
peroxidation, and anti- and pro-oxidant enzyme systems in
enterocytes across the crypt–villus axis in intestine.
Methods Wistar rats (90–100 g) were administered 1 mL of
30% ethanol daily for 39 days. Intestinal epithelial cells were
isolated in fractions. Malondialdehyde levels, and activities
of glutathione-S-transferase (GST), glutathione reductase
(GR), superoxide dismutase (SOD) and catalase were
determined in various cell fractions. Incorporation of H3-
thymidine into DNA of enterocytes was also determined.
Results Lipid peroxidation was elevated by two- to threefolds
in both villus and crypt cells in ethanol-fed animals compared
to controls. The activities of GST and GR were four- to
six-folds higher in villus tip cells compared to crypt base cells.
The activities of SOD and catalase were five- to seven-fold
higher in crypt base cells compared to villus tip cells. Ethanol
feeding elevated the activities of SOD (76-190%) and catalase
(20-150%) in enterocytes all along the crypt–villus axis compared
to the controls. H3 thymidine incorporation into DNA
of enterocytes was reduced by half in ethanol-fed rats compared
to controls.
Conclusions There is a gradient in the concentration of lipid
peroxides in enterocytes across the crypt–villus axis, being high at the villus tip and low at the crypt base in the rat intestine. Ethanol feeding enhanced lipid peroxidation in both villus and crypt cells.
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Keywords
Chronic alcoholism, lipid peroxidation, rat intestine
Citation
Kalra Amandeep Kaur, Gupta Shiffalli, Turan Aasma, Mahmood Safrun, Mahmood Akhtar. Ethanol-induced changes in lipid peroxidation of enterocytes across the crypt–villus axis in rats. Indian Journal of Gastroenterology. 2010 Jan-Feb; 29(1): 23-27.