Detection of dengue virus 4 from central India.

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dc.contributor.authorBarde, P V
dc.contributor.authorGodbole, S
dc.contributor.authorBharti, P K
dc.contributor.authorChand, Gyan
dc.contributor.authorAgarwal, M
dc.contributor.authorSingh, Neeru
dc.date.accessioned2013-01-29T05:26:39Z
dc.date.available2013-01-29T05:26:39Z
dc.date.issued2012-09
dc.description.abstractBackground & objectives: Dengue is an important arboviral disease. All four dengue virus serotypes are reported to be circulating in India. It is also known that different serotypes, genotypes and clades of genotype determine outbreak severity. Dengue affected children are known to have serious disease outcome. We carried out this study to give reliable diagnosis of dengue infection in children and to detect circulating serotype in central India. Methods: Samples collected from paediatric patients suspected to have dengue fever were subjected to IgM and IgG ELISA to determine dengue virus infection. Samples collected within 0-5 days of onset of illness and positive by IgM ELISA were tested by nested reverse transcription polymerase chain reaction (nRT-PCR). The PCR products were sequenced and analyzed. Results: Of the 89 samples tested, 18 and 7 were positive for dengue IgM and IgG, respectively. Dengue activity was observed in both Jabalpur city and adjoining rural settings. One sample found positive by nRT-PCR was further sequenced to confirm dengue virus 4 as aetiological agent. Interpretation & conclusions: Our findings demonstrated dengue virus infection in children and adolescent in central India. Because of continuous changing epidemiology, it is important to monitor dengue virus activity at both serological and molecular level in this part of the country for better patient care and management.en_US
dc.identifier.citationBarde P V, Godbole S, Bharti P K, Chand Gyan, Agarwal M, Singh Neeru. Detection of dengue virus 4 from central India. Indian Journal of Medical Research. 2012 Sept; 136(3): 491-494.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/144774
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510898/en_US
dc.subjectCentral Indiaen_US
dc.subjectdengueen_US
dc.subjectdengue virus type 4en_US
dc.subjectIgG IgM ELISAen_US
dc.subjectpaediatric patientsen_US
dc.subject.meshChild
dc.subject.meshChild, Preschool
dc.subject.meshDisease Management
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methods
dc.subject.meshDengue --epidemiology
dc.subject.meshDengue --virology
dc.subject.meshDengue Virus --genetics
dc.subject.meshDengue Virus --isolation & purification
dc.subject.meshHumans
dc.subject.meshInfant
dc.subject.meshInfant, Newborn
dc.subject.meshImmunoglobulin G
dc.subject.meshImmunoglobulin M
dc.subject.meshIndia --epidemiology
dc.titleDetection of dengue virus 4 from central India.en_US
dc.typeArticleen_US
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