Effect of bevacizumab (Avastin™) on mitochondrial function of in vitro retinal pigment epithelial, neurosensory retinal and microvascular endothelial cells.

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dc.contributor.authorLuthra, Saurabh
dc.contributor.authorSharma, Ashish
dc.contributor.authorDong, Joyce
dc.contributor.authorNeekhra, Aneesh
dc.contributor.authorGramajo, Ana L
dc.contributor.authorSeigel, Gail M
dc.contributor.authorKenney, M Cristina
dc.contributor.authorKuppermann, Baruch D
dc.date.accessioned2015-01-24T05:45:07Z
dc.date.available2015-01-24T05:45:07Z
dc.date.issued2013-12
dc.description.abstractPurpose: To evaluate the effect of bevacizumab on the mitochondrial function of human retinal pigment epithelial (ARPE‑19), rat neurosensory retinal (R28) and human microvascular endothelial (HMVEC) cells in culture. Materials and Methods: ARPE‑19 and R28 cells were treated with 0.125, 0.25, 0.50 and 1 mg/ml of bevacizumab. The HMVEC cultures were treated with 0.125, 0.25, 0.50 and 1 mg/ml of bevacizumab or 1 mg/ml of immunoglobulin G (control). Mitochondrial function assessed by mitochondrial dehydrogenase activity (MDA) was determined using the WST‑1 assay. Results: Bevacizumab doses of 0.125 to 1 mg/ml for 5 days did not significantly affect the MDA of ARPE‑19 cells. Bevacizumab treatment at 0.125 and 0.25 mg/ml (clinical dose) did not significantly affect the MDA of R28 cells; however, 0.50 and 1 mg/ml doses significantly reduced the R28 cell mitochondrial function. All doses of bevacizumab significantly reduced the MDA of proliferating and non‑proliferating HMVEC. Conclusion: Bevacizumab exposure for 5 days was safe at clinical doses in both ARPE‑19 and R28 retinal neurosensory cells in culture. By contrast, bevacizumab exposure at all doses show a significant dose‑dependent decrease in mitochondrial activity in both the proliferating and non‑proliferating HMVEC in vitro. This suggests a selective action of bevacizumab on endothelial cells at clinical doses.en_US
dc.identifier.citationLuthra Saurabh, Sharma Ashish, Dong Joyce, Neekhra Aneesh, Gramajo Ana L, Seigel Gail M, Cristina Kenney M, Kuppermann Baruch D. Effect of bevacizumab (Avastin™) on mitochondrial function of in vitro retinal pigment epithelial, neurosensory retinal and microvascular endothelial cells. Indian Journal of Ophthalmology. 2013 Dec ; 61 (12): 705-710.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/155474
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3917387/en_US
dc.subjectBevacizumaben_US
dc.subjectmitochondrial functionen_US
dc.subjectmicrovascular endothelial cellsen_US
dc.subjectneurosensory retinal cellsen_US
dc.subjectretinal pigment epithelial cellsen_US
dc.subjecttissue cultureen_US
dc.titleEffect of bevacizumab (Avastin™) on mitochondrial function of in vitro retinal pigment epithelial, neurosensory retinal and microvascular endothelial cells.en_US
dc.typeArticleen_US
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