Purification and physicochemical studies of human plasma thiol proteinase inhibitors.

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dc.contributor.authorDas Gupta, Ren_US
dc.contributor.authorBano, Ben_US
dc.contributor.authorGhosh, P Ken_US
dc.date.accessioned1998-02-12en_US
dc.date.accessioned2009-05-27T09:11:50Z
dc.date.available1998-02-12en_US
dc.date.available2009-05-27T09:11:50Z
dc.date.issued1998-02-12en_US
dc.description.abstractThe physicochemical properties of thiol proteinase inhibitors (TPI) isolated from outdated human blood have been studied. A simple technique which includes ammonium sulphate precipitation, Sephadex G-200 gel filtration and ion-exchange chromatography led to the isolation of 4 isolates namely TPI-1, TPI-2, TPI-3 and TPI-4 having molecular mass of 70, 155, 195 and 497 kDa respectively. The latter two forms are the new isolates unreported previously. They exhibit similar pH stability, inhibition spectra with papain, cathepsin B and trypsin, antigenic properties and glycoprotein nature. The TPI-4, however, was found to be most heat stable showing no decrease in inhibitory activity when heated upto 70 degrees C for 30 min. Our work suggests that TPI-3 and TPI-4 are the oligomers of TPI-1.en_US
dc.description.affiliationDepartment of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India.en_US
dc.identifier.citationDas Gupta R, Bano B, Ghosh PK. Purification and physicochemical studies of human plasma thiol proteinase inhibitors. Indian Journal of Biochemistry & Biophysics. 1998 Feb; 35(1): 21-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26762
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshBlood Preservationen_US
dc.subject.meshChemistry, Physicalen_US
dc.subject.meshCysteine Proteinase Inhibitors --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshPhysicochemical Phenomenaen_US
dc.titlePurification and physicochemical studies of human plasma thiol proteinase inhibitors.en_US
dc.typeJournal Articleen_US
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