Standardization of fungal polymerase chain reaction for the early diagnosis of invasive fungal infection.

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dc.contributor.authorDeshpande, P
dc.contributor.authorShetty, A
dc.contributor.authorMehta, A
dc.contributor.authorKapadia, F
dc.contributor.authorHedge, A
dc.contributor.authorSoman, R
dc.contributor.authorRodrigues, C
dc.date.accessioned2012-12-14T09:39:58Z
dc.date.available2012-12-14T09:39:58Z
dc.date.issued2011-10
dc.description.abstractBackground: An early initiation of antifungal therapy in invasive fungal infections (IFIs) is critical in reducing the high mortality rate. Current diagnosis of fungal infection relies on microscopy, culture, antigen, antibody specific tests and histological diagnosis. However, these tests either lack sensitivity or specificity. There is thus the need for a rapid, specific and accurate diagnostic method. Objective: The aim of our study was to establish PCR for the rapid detection of Candida and Aspergillus species in clinical specimens with improved sensitivity and specificity. Materials and Methods: A total of 71 proven cases of IFI (confirmed by culture) were collected. A total of 15 healthy, 15 patients suffering from bacterial sepsis and 15 patients with HIV, HBV viral infections were included as controls. Clinical specimens were subjected to a standardized nested amplification to produce Round I (504 bp) and Round II (150 bp) amplicons. Restriction digestion was performed on these products for further identification. Results: Analytical sensitivity was determined using 10 6 -10 CFU/ml of cell suspension. The lower detection limit of the assay was 10 CFU/ml of blood. This test was 100% sensitive and specific with a positive predictive value of 100% and a negative predictive value of 96.7%. Conclusion: The assay was found to be effective for the rapid detection of Candida and Aspergillus in clinical specimens.en_US
dc.identifier.citationDeshpande P, Shetty A, Mehta A, Kapadia F, Hedge A, Soman R, Rodrigues C. Standardization of fungal polymerase chain reaction for the early diagnosis of invasive fungal infection. Indian Journal of Medical Microbiology. 2011 Oct-Dec; 29(4): 406-410.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143865
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2011;volume=29;issue=4;spage=406;epage=410;aulast=Deshpandeen_US
dc.subjectAspergillusen_US
dc.subjectCandidaen_US
dc.subjectnested polymerase chain reactionen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectrestriction enzyme digestionen_US
dc.subject.meshAspergillosis --diagnosis
dc.subject.meshAspergillus --genetics
dc.subject.meshAspergillus --isolation & purification
dc.subject.meshCandida --genetics
dc.subject.meshCandida --isolation & purification
dc.subject.meshCandidiasis --diagnosis
dc.subject.meshEarly Diagnosis
dc.subject.meshFungemia --diagnosis
dc.subject.meshHumans
dc.subject.meshMolecular Diagnostic Techniques --methods
dc.subject.meshMolecular Diagnostic Techniques --standards
dc.subject.meshMycology --methods
dc.subject.meshMycology --standards
dc.subject.meshPolymerase Chain Reaction --methods
dc.subject.meshPolymerase Chain Reaction --standards
dc.subject.meshSensitivity and Specificity
dc.titleStandardization of fungal polymerase chain reaction for the early diagnosis of invasive fungal infection.en_US
dc.typeArticleen_US
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