Effects of heparin on thalassemia genes analysis by PCR

Simple item page
dc.contributor.authorKitsada Singha; Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorSirintra Chaitongyot; Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorGoonnapa Fucharoen; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.contributor.authorSupan Fucharoen; Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen Universityen_US
dc.date.accessioned2011-02-20T04:32:57Z
dc.date.available2011-02-20T04:32:57Z
dc.date.created2010-04-11en_US
dc.date.issued2010-04-11en_US
dc.description.abstractEDTA-anticoagulated blood is usually recommended for use in routine analysis of thalassemia genes. However, collection of blood from the fetus or newborns is usually done using heparin because of its more potent anti-coagulant activity. However, since heparin can inhibit Taq polymerase enzyme used in PCR, the use of heparinized blood for PCR analysis may be affected. ACD, another blood anti-coagulant with a preservative property may be used as an alternative anti-coagulant. In this study, the effects of EDTA, heparin and ACD anti-coagulants on PCR analysis of a – thalassemia 1 (SEA deletion) and bE – globin gene were compared. a – thalassemia 1 was detected using gap – PCR and bE – globin gene was identified by allele specific PCR assay. It was found that both EDTA and ACD anti-coagulants had no effect on the efficiency of PCR analysis of the two thalassemia genes. However, substantial reduction in the efficiency of PCR analysis was observed with the use of heparin as an anti-coagulant at a concentration over 25 IU/ml. Amplification efficiency was improved when heparinized blood DNA was diluted prior to PCR analysis. The result from this study should prove useful for development of a guideline of blood collection for routine PCR analysis of halassemia.                              en_US
dc.identifier.citationJournal of Medical Technology and Physical Therapy; Volume 22 Number 1, January - April 2010; 45-50en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/130897
dc.language.isoen_USen_US
dc.publisherKhon Kaen Universityen_US
dc.rightsKhon Kaen Universityen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/article/view/3379en_US
dc.titleEffects of heparin on thalassemia genes analysis by PCRen_US
dc.typeORIGINAL ARTICLEen_US
Files
Collections
Journal of Medical Technology and Physical Therapy