A murine model of allergy caused by American cockroach (CR), Periplaneta americana.

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dc.contributor.authorSookrung, Nitaten_US
dc.contributor.authorIndrawattana, Nitayaen_US
dc.contributor.authorTungtrongchitr, Anchaleeen_US
dc.contributor.authorKaruhassuwan, Charanen_US
dc.contributor.authorChaisri, Uraien_US
dc.contributor.authorChaicumpa, Wanpenen_US
dc.date.accessioned2009-05-27T17:18:29Z
dc.date.available2009-05-27T17:18:29Z
dc.date.issued2008-12-06en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractAn animal model resembling the human immuno-pathological features of CR allergy is needed for CR allergy research, e.g., measuring allergenicity of novel allergens, testing immunotherapeutic efficacies of drugs and vaccines. In this study we develop a murine model of American CR, P. americana allergy. BALB/c mice, 6 weeks old, were individually intraperitoneally injected with three doses (days 0, 7 and 14) of alum adjuvanted-crude extract of P. americana. On days 21 and 23, they were given crude CR extract in PBS intranasally (10 microl) and aerosolically (10 ml) via an air-pressure nebulizer, respectively. Mice received alum alone and PBS instead of the CR extract served as non-allergenic controls. All mice were bled twenty four hours after the nebulization and sacrificed. Their serum samples, broncho-alveolar lavage fluids (BALF), and lung tissues were collected. BALF of all allergen-treated mice had marked cellular infiltration notably neutrophils, eosinophils and lymphocytes. The average total cell count in BALF of the allergenic mice was 1.9 x 10(5) cells/ml which out-numbered those of the non-allergenic controls (8 x 10(4) cells/ml). The eosinophil infiltration was pronounced in lungs of the allergen-treated mice. Specific serum IgE to the CR extract elevated in serum samples of all allergen treated mice and nil in the sera of the controls. None of the mice showed detectable level of IgG2a to the CR extract. RT-PCR revealed that all allergen-treated mice had marked increase of IL-13, IL-4 and TNF-alpha gene expressions, slight increase of IL-5 gene expression, and absence of detectable IFN-gamma gene expression in comparison to the non-allergenic controls. None of the allergen-treated mice and 50% of the non-allergenic controls had IL-12 gene expression as detected by RT- PCR. One allergen treated-mouse (25%) had subpar level of the IL-18 gene expression compared to the controls. Results of the quantitative real-time PCR conformed to those of the RT-PCR. A murine model of P. americana resembling human allergic manifestations was successfully developed.en_US
dc.description.affiliationOffice for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. sinsru@mahidol.ac.then_US
dc.identifier.citationSookrung N, Indrawattana N, Tungtrongchitr A, Karuhassuwan C, Chaisri U, Chaicumpa W. A murine model of allergy caused by American cockroach (CR), Periplaneta americana. Asian Pacific Journal of Allergy and Immunology. 2008 Jun-Sep; 26(2-3): 143-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36906
dc.language.isoengen_US
dc.subject.meshAllergensen_US
dc.subject.meshAlum Compoundsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Movementen_US
dc.subject.meshComplex Mixtures --administration & dosageen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshEosinophils --immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshHypersensitivity --blooden_US
dc.subject.meshImmunization, Secondaryen_US
dc.subject.meshInjections, Intraperitonealen_US
dc.subject.meshLeukocyte Counten_US
dc.subject.meshLung --immunologyen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshPeriplaneta --immunologyen_US
dc.titleA murine model of allergy caused by American cockroach (CR), Periplaneta americana.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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